中国药理学通报2012，Vol.28Issue(9)：1307-1310,4.DOI:10.3969/j.issn.1001-1978.2012.09.028
不同纯度高良姜素的药代动力学比较研究
A comparative study of pharmacokinetics of different purity galangin
摘要
Abstract
Aim To investigate the pharmacokinetics of different purity galangin( GL )in rats in vivo. Methods SD rats were used throughout the experiment and their body weights ranged from 240 to 300 g. All of them were distributed randomly to different test groups ( 6 rats per group ). GL was administered at a single dose via tail vein. The blood was collected from the tail vein plexus at different time points. A reversed-phase high performance liquid chromatography ( RP-HPLC ) method, in which the external standard method was used, was developed to determine concentrations of GL in rats. The main parameters of pharmacokinetics were calculated by DASS 2. 0 software. Results Under the established conditions of HPLC, GL had a validated quantitation ranging from 0. 05 mg ·L-1 to 1. 8 mg · L -1. The lower limit of detection ( LLOD ) in plasma was 0. 03 mg · L-1( S/N =3 ). At the concentrations of 0. 24, 0. 47, 0. 94 mg · L-1, absolute recovery were ranged from 75. 5% to 86. 9% , relative recovery were ranged from 85. 8% to 91. 2%. The RSD of the prec- ision intra-and inter-day were less than 5. 0%( n=5). The concentration-time course of GL was best fitted to a two-compartment open model after a single oral administration dose of 100 mg · kg-1, which the main phar-macokinetic parameters T2/1α, T2/1β , Ka, AUC, Cl/F and V/F were 19.415 min, 33.983 min, 0.059 min-1,101.722 mg · min · L-1,0.983 L · min-1 · kg-1 and 5. 073 L · kg-1 for GL of 90% ;24. 398 min, 31.719 min,0.048 min-1,55. 201 mg · min · L-1, 1. 812 L · min-1 · kg-1 and 6. 861 L · kg-1 for GL of 99% , respectively. Conclusion The developed method is shown to be simple, accurate and rapid, and can satisfy the requirement of pharmacokinetic study of GL in rat. GL at a high purity is absorbed more slowly and eliminated faster than that at a relatively low purity after a single oral administration dose of 100 mg ·kg-1 in rats.关键词
高良姜素/高良姜/反相高效液相色谱法/外标法/药代动力学/大鼠Key words
galangin/ galangal/ revensed-phase high performance liquid chromategraphy/ external standard method/ pharmacokinetics/ rat分类
医药卫生引用本文复制引用
李建梅,霍仕霞,高莉,吴培培,彭晓明,林娟,闫明..不同纯度高良姜素的药代动力学比较研究[J].中国药理学通报,2012,28(9):1307-1310,4.基金项目
国家自然科学基金资助项目 (81160556) （81160556）
