中国药理学通报2012，Vol.28Issue(9)：1311-1314,4.DOI:10.3969/j.issn.1001-1978.2012.09.029
不同剂量地塞米松对大鼠松质骨代谢的影响
Effects of different doses of dexamethasone on trabecular bone metabolism in rat
摘要
Abstract
Aim To investigate and study the effects of different doses of dexamethasone on trabecular bone metabolism of rat. Methods Thirty-one SPF SD rats were randomly divided into normal control group, dexamethasone 1 mg · kg-1, 2. 5 mg · kg-1, and 5 mg · kg-1 groups. They were intravenously injected with dexamethasone twice per week. 8 weeks later, all rats were killed and their proximal tibia metaphysis( PTM ) and the fourth lumbars were processed with undecalci-fied sections for bone histomorphometry analysis. Results Compared with normal group, percent trabecular bone area of PTM increased in 2. 5 mg and 5 mg groups. But percent labeled perimeter, bone formation rate and percentage of osteoclast perimeter of three dexamethasone groups decreased remarkably. And the percentage of osteoblast perimeter decreased in 5mg groups only. Histomorphometric static data of lumbar vertebra displayed that there was no statistic difference in percent trabecular bone area in all dexamethasone groups, but the four dynamic parameters % L. Pm, BFR,%Ob. Pm and %Oc. Pm significantly reduced in these groups. Conclusions Dexamethasone maybe have a positive effect on bone metabolism in the beginning and increase the bone mass of PTM. But it soon inhibits the bone formation dose-dependently and inhibits the bone resorption as well after 8 weeks. Dexamethasone inhibits trabecular bone metabolism in both PTM and lumbar.关键词
糖皮质激素/地塞米松/松质骨/骨代谢/骨组织形态计量学/大鼠Key words
glucocorticoid/ dexamethasone/ trabecular bone/bone metabolism/ bone histomorphometry/rat分类
医药卫生引用本文复制引用
陈艳,黄连芳,刘衍志,陈文双,朱觉新,崔燎..不同剂量地塞米松对大鼠松质骨代谢的影响[J].中国药理学通报,2012,28(9):1311-1314,4.基金项目
国家自然科学基金资助项目(No 30973574) （No 30973574）
