中国药理学通报2012，Vol.28Issue(9)：1319-1324,6.DOI:10.3969/j.issn.1001-1978.2012.09.031
基于中医辨证用药特点建立大鼠血热出血模型
Establishment of blood-heat and hemorrhage syndrome rat model based on Traditional Chinese Medicine characteristics
摘要
Abstract
Aim To establish and contrast two kinds of rat models of blood-heat and hemorrhage syndrome based on Traditional Chinese Medicine characteristics. Methods In model Ⅰ , rats were fed with the water decoction of Rhizoma Zingiberis( RZD ) at the dose of 15 g · kg-1 and 5% alcohol for 14 days continuously, and the rats in control group I were fed with water in the same volume. In model Ⅱ , rats were injected of dry yeast solutions subcutaneously at the dose of 2 g · kg-1 and fed with 5% alcohol, and the physiological saline was given to the control group Ⅱ in the same volume. The amount of body weight, daily diet, volume of daily drinking and urine, pellet weight and its moisture capacity, rectal temperature were recorded. After 14 days, thrombin time( TT ), fibrinogen content( FIB ), prothrom-bin time( PT ), activated partial thromboplastin time( APTT ), red blood cell( RBC ), hemoglobin( HGB ), hematocrit( HCT ) and hemorheological parameters were examined, and the histo-logical changes of tongue, lung, and stomach were observed by hematoxylin-eosin stain. Results After 14 days, the amount of body weight, rectal temperature and daily diet, the volume of daily drinking and urine of model Ⅰ increased ( P < 0. 05, P < 0. 01 ), while the pellet weight and its moisture capacity decreased ( P < 0. 01 ) with the comparison of control group I . Compared with control group Ⅱ , the rectal temperature of model II was upgraded obviously( P < 0. 01 ) four hours after the injection. Both model groups had significant difference compared with the control groups, the blood and plasma viscosity, RBC, HGB, HCT, TT, APTT and FIB were prolonged, ( P < 0. 05, P < 0. 01 )andPT was shortened( P <0. 01 ). Vasodilatation in the tongue and stomach, and hemorrhage in the lung were also observed in the two model groups. Conclusion Giving a large dose of RZD orally or injecting dry yeast solutions subcutaneous-ly can induce vasodilatation, hemorrhage, dysfunction of hemor-rheology and coagulation system in SD rats, and modeling with RZD is more appropriate to describe the blood-heat and hemor- rhage syndrome based on traditional Chinese medicine characteristics.关键词
大鼠/血热出血/干姜/干酵母/凝血功能/血液流变学/肺出血Key words
rat/ blood-heat and hemorrhage syndrome/ Rhizoma Zingiberis/ dry yeast/ coagulation function/ hemorheology/ pulmonary hemorrhage分类
医药卫生引用本文复制引用
柳佳,张丽,丁安伟,姚映芷,单鸣秋,喻斌,姚卫峰..基于中医辨证用药特点建立大鼠血热出血模型[J].中国药理学通报,2012,28(9):1319-1324,6.基金项目
国家自然科学基金立项项目(No 81173547) （No 81173547）
