中国油料作物学报2012，Vol.34Issue(3)：233-238,6.
芸薹种UGMS标记及抗根肿病基因连锁标记的定位
Mapping of unigene - derived microsatellite and markers linked to clubroot resistance loci in Brassica rapa
摘要
Abstract
To localize the previously developed unigene - derived microsatellite (UGMS) markers, genomic SSR markers and markers linked to the clubroot resistance ( CR) loci in Brassica rapa, a genetic linkage map was constructed using a backcross population of Chinese cabbage 59-1 and CR turnip WJ04. Clubroot resistance test indicated that WJ04 had dominant resistance to four races of No. 2,4,7 and 10 of Plasmordiam brassicase, while 59 -1 was susceptible to all of the races. Localization results showed that the map contained 59 UGMS, 72 genomic SSR and 4 markers linked to 4 different CR loci, covering a genetic distance of 1 116cM of B. rapa genome. Four markers were linked to Crr1 ,Crr2,Crr3 and CRb genes respectively. Polymorphism frequency of UGMS markers was 30. 1% between the two subspecies of Chinese cabbage and turnip, which was lower than that of the genomic SSR (50.8%).关键词
芸薹种/单一基因微卫星标记/根肿病/遗传连锁图谱/遗传距离Key words
Brassica rapa/ Unigene — derived microsatellite marker/ Clubroot disease/ Genetic linkage map/ Genetic distance分类
农业科技引用本文复制引用
敬静,陈静静,张椿雨,朴钟云..芸薹种UGMS标记及抗根肿病基因连锁标记的定位[J].中国油料作物学报,2012,34(3):233-238,6.基金项目
国家自然科学基金(31171967) （31171967）
辽宁省自然科学基金(20102199) （20102199）
教育部高等学校博士学科点专项科研基金(20092103110006) （20092103110006）
