中国医学科学院学报2012，Vol.34Issue(3)：244-248,5.DOI:10.3881/j.issn.1000-503X.2012.03.010
一种肿瘤相关抗原在食管鳞状细胞癌中的鉴定与表达
Identification and Expression of A Tumor-associated Antigen in Esophageal Squamous Cell Carcinoma
高红军 1岳志刚 1郑敏 1郑朝旭2
作者信息
- 1. 煤炭总医院检验科,北京100028
- 2. 中国医学科学院北京协和医学院肿瘤医院腹部外科,北京100021
- 折叠
摘要
Abstract
Objective To search for novel tumor associated antigens (TAA) in esophageal squamous cell carcinoma ( ESCC). Methods The proteins extracted from tissues of ESCC were separated by two dimensional polyacrylamide gel electrophoresis and transferred to PVDF membrane. Sera from ESCC patients and healthy individuals were used for primary antibodies for Western blot analysis. The differential spots were excised for trypsin hydrolysis and the tryptic peptides were analyzed by matrix-assisted laser disorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The identified TAA of ESCC was validated by immunohis-tochemical staining (IHC). Results Sera from ESCC patients yielded multiple positive spots, and one 28 800 Da protein that exhibited positive reactivity with 60% (12/20) sera of ESCC patients and only 5% (1/20) sera of healthy controls (P <0. 01). The 28 800 Da protein was identified as phosphoglycerate mutase 1 (PGAM1) by MALDI-TOF-MS. Immunohistochemical analysis showed that PGAM1 was located in both cytoplasm and nucleus, and had a higher expression in cancer tissues. Conclusion PGAM1 maybe a candidate of ESCC.关键词
肿瘤相关抗原/食管鳞状细胞癌/磷酸甘油酸变位酶1/表达/鉴定Key words
tumor associated antigen/ esophageal squamous cell carcinoma/ phosphoglycerate mutase 1/ expression/ identificaton分类
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高红军,岳志刚,郑敏,郑朝旭..一种肿瘤相关抗原在食管鳞状细胞癌中的鉴定与表达[J].中国医学科学院学报,2012,34(3):244-248,5.
