中国中医药信息杂志2012，Vol.19Issue(8)：52-54,3.DOI:10.3969/j.issn.1005-5304.2012.08.019
HPLC-ELSD测定肉苁蓉及管花肉苁蓉中半乳糖醇含量
Determination of Galactitol in Cistanche deserticola and Cistanche tubulosa by HPLC-ELSD
摘要
Abstract
Objective To establish a method for the determination of galactitol in Cistanche deserticola and Cistanche tubulosa, and to provide evidence for the quality control of Cistanches Herba. Methods The contents of galactitol in 20 batches samples of Cistanche deserticola and Cistanche tubulosa were determined by HPLC-ELSD. The samples were separated by Alltech Pevail Carbohydrate ES column (4.6 mm×250 mm, 5 μm) with acetonitrile-water (80∶20) as mobile phase. The flow rate was 1.0 mL/min and the injection volume was 10 μL. Shift tube temperature of ELSD was set at 80 ℃ and the gas flow rate was 3.0 L/min. Results The linear range of galactitol was 89.6－1 120 mg/L (r＝0.999 0), method detection limit was 15.998 mg/L, limit of quantitation was 79.903 mg/L. The average recovery rate of galactitol in Cistanche deserticola and Cistanche tubulosa was 100.34% and 101.11% respectively with RSD of 1.72% and 1.33 % (n＝5). Conclusion The method is sensitive, repeatable and accurate, which can be used for the quality control of Cistanches Herba.关键词
肉苁蓉/管花肉苁蓉/高效液相色谱-蒸发光散射检测器/半乳糖醇Key words
Cistanche deserticola；Cistanche tubulosa；HPLC-ELSD；galactitol分类
医药卫生引用本文复制引用
赵奎君,梁丽娟,毕丹,董婷霞,詹华强..HPLC-ELSD测定肉苁蓉及管花肉苁蓉中半乳糖醇含量[J].中国中医药信息杂志,2012,19(8):52-54,3.基金项目
首都医学发展基金(中医药类,JJ2009-30) （中医药类,JJ2009-30）
