摘要
Abstract
Objective To study the difference of S-TKl level among patients with breast cancer, patients with benign breast tumor and healthy volunteers, to investigate the correlation between S TK1 level and staging,the size of tumor,grade,ER,PR or HER-2 in patients with breast cancer and analyze the mechanism that S-TKl involved into the recurrence and metastasis of breast cancer patients. Methods The S-TK1 levels of breast cancer,breast benign tumor and healthy volunteers were detected by enhanced chem-iluminescence(ECL) dot blot assay. Results (l)The concentrations of S-TKl in patients with breast cancer before treatment (group A), benign breast tumor (group B)(17. 98 ± 10. 20)pmol/L,and healthy volunteersCgroup C)(l, 50 ± 1. 02)pmol/L and (0. 88 + 0. 45)pmol/L, respectively. There was significant difference between group A and group B or group C(P<0. 001, P<0. 001) , and between group B and group C(F = 0. 017). Also in group A the concentration of S-TKl of staging Ⅲ was significantly higher than that of staging Ⅱ ,(21. 54 ± 11. 85)pmol/L vs. = (14.05 + 6. 19)pmol/L(P = 0. 018). (2)There was a positive correlation between the concentration of S-TKl with grade, HER-2 and the size of tumor (r = 0. 596,0. 324,0. 397, P<0. 001, P = 0. 042, P = 0. 011, respectively) and a negative correlation between ER and the concentration of S-TKl (r = - 0. 391 ,P = 0. 013). But the concentration of S-TKl had correlation with PR(P= 0. 395,r = - 0. 138); (3)The S-TKl level in patients with recurrence and metastasis before therapy was higher than that in disease-free patients, (23. 17 ± 13. 15) pmol/L vs. (14. 31 ± 5. 50) pmol/L(P = 0.011).The DFS in low and middle S-TKl level was longer than that in high S-TK1 level, but there was not significant diference(P= 0. 138), Conclusion S-TKl perhaps could be one of markers for early breast cancer diagnosis. The level of S-TKl has relation with the progress of breast cancer,and results into recurrence and metastasis.关键词
胸苷激酶-1/乳腺癌/化学发光点印迹Key words
Thymidine kinase-1 /Breast cancer / Enhanced chemiluminescence dot blot assay分类
医药卫生
