肿瘤防治研究2012，Vol.39Issue(6)：658-661,4.DOI:10.3971/j.issn.1000-8578.2012.06.011
Rock2对肝癌细胞放疗敏感度的影响
Effect of Rock2 on Radiation Sensitivity of Hepatocellular Carcinoma Cells
摘要
Abstract
Objective To investigate the effects of Rock2 on radiation sensitivity of heparocellular carcinoma cells(HCC). Methods The expression of Rock2 in HepG2 and Huh-7 cells was decreased and DNA damage model was constructed by IR exposure. MTT assay was used to detect cell proliferation. The change of Cdc25A expression was measured by Western blot and Cdk2/CyclinE activity was detected by fluorescence resonance energy transfer. Results shRock2 cells exposed to IR(to induce DNA damage) showed significantly decreased survival,compared with siControl cells. Westcrn blot analysis revealed that when exposed to IR,Cdc25A decreased more obviously in shRock2 cells as compared to siControl cells. After exposure to IR,the decreased in Cdk2/Cyclin E activity was more apparent in shRock2 cells compared to the siControl cells. Conclusion Inhibition of Rock2 expression could enhance the radiation sensitivity of HCC by suppressing Cdc25A. Rock2 might be a new target gene for treatment of HCC.关键词
Rock2/肝细胞癌/Cdc25A/放疗Key words
Rock2/hepatocellular carcinoma /Cdc25 A /radiotherapy分类
医药卫生引用本文复制引用
刘天德,余新,刘秀霞,袁荣发,蒋成行,邵江华..Rock2对肝癌细胞放疗敏感度的影响[J].肿瘤防治研究,2012,39(6):658-661,4.基金项目
国家自然科学基金资助项目(30860272) （30860272）
江西省研究生创新基金资助项目(YC10A018) （YC10A018）
