癌变·畸变·突变2012，Vol.24Issue(4)：283-285,289,4.DOI:10.3969/j.issn.1004-616x.2012.04.010
硝基苯和苯胺对大鼠原代肝细胞氧化损伤的作用
Combined toxicity of nitrobenzene and aniline in primary culture of rat hepatocytes
摘要
Abstract
OBJECTIVE: To study the combined toxicity of nitrobenzene and aniline on primary culture hepatocyte of Wistar rats. METHODS: Nitrobenzene and aniline alone or combined were used to treat the cells for 24 h, and then MTT method was used to measure the survival rate of the cells. Kits were used to measure the MDA, ALT, SOD, GSH and GSH-px levels in the cells or supernatant. RESULTS: After treating with different concentrations of nitrobenzene and aniline alone or combined for 24 h, the survival rate of the cells was significantly lower than that of the cells in control group, with a dose-response relationship. After treating with 10 and 20 mmol/L nitrobenzene and aniline alone or combined for 24 h, the cell MDA and ALT levels of all groups were significantly higher than that of control group (P<0.05). The cell SOD, GSH and GSH-px levels in groups were significantly lower than that of control group (P<0.05). There were significant differences of MDA, ALT, SOD, GSH and GSH-px levels between the combined treatment group and those treated with nitrobenzene or aniline(P<0.05). CONCLUSION: Nitrobenzene and aniline alone or combined may lead to synergistic toxicity effect on hepatocytes. Further research is needed for study.关键词
硝基苯/苯胺/联合毒性/氧化损伤Key words
nitrobenzene/aniline/combined toxicity/oxidative damage分类
医药卫生引用本文复制引用
贾莉,王彦军,董淑英,商蕾,那晓琳..硝基苯和苯胺对大鼠原代肝细胞氧化损伤的作用[J].癌变·畸变·突变,2012,24(4):283-285,289,4.基金项目
哈尔滨医科大学医学基础学科青年科学基金项目(060001) （060001）
