现代农业科技Issue(16)：133-134,141,3.
烟草黑胫病菌拮抗细菌的筛选与鉴定
Screening and Identification of Antagonistic Bacteria Against Phytophthora parasitica var. nicotianae
摘要
Abstract
Totally 107 bacterial strains were isolated from different tobacco rhizosphere soils,of which 13 strains showed antagonistic activity against phytophthora parasitica var.nicotianae.LZ-7 strain had the best room flat antibacterial activity and inhibition zone diameter reached 27.82 mm.Pot experiment showed that the LZ-7 and A3 had better control effect which reached 59.12% and 55.84%.The 16S rDNA fragment was amplified from strain LZ-7 by PCR,and the 16S rDNA sequence analysis indicated that strain LZ-7 shared 99% 16S rDNA sequence homology with Bacillus pumilus.In the phylogenetic framework of bacterial classification,LZ-7 belonged to Bacillus pumilus.关键词
烟草黑胫病/拮抗细菌/生物防治/筛选/鉴定Key words
Phytophthora parasitica var.nicotianae/antagonistic bacteria/biological control/screening/identification分类
农业科技引用本文复制引用
王栋,伏晓,韩新宇,罗定棋,何余勇,张永辉,雷晓..烟草黑胫病菌拮抗细菌的筛选与鉴定[J].现代农业科技,2012,(16):133-134,141,3.基金项目
泸州市烟草公司科技项目 （）
