安徽农业大学学报2012，Vol.39Issue(4)：497-501,5.
油茶AFLP反应体系建立及其在遗传多样性研究上的应用
Establishment of AFLP reaction system and its application to analysis of genetic diversity for oil tea (Camellia olifera Abel.)
摘要
Abstract
Amplified fragment length polymorphism (AFLP) reaction system was established for Camellia oleifera Abel.. Four AFLP primer pairs were tested and selected. The genetic diversity was analyzed based on the primer combinations. A total of 23 loci with unambiguous amplified fragments were revealed per primer pair with a number of polymorphic loci (N) of 18, a percentage of polymorphic loci (PPL) of 79.13%, an effective number of alleles per locus (ne) of 1.320, an expected heterozygosity (He) of 0.184, and Shannon's information index (I) of 0.267. The results indicate that AFLPs are suitable markers for the research of C. olifera, trees of the species possess a relatively high level of genetic diversity. The present study provids theoretic and technical support for the further population genetic study of C. olifera.关键词
AFLPs/油茶/遗传多样性/PCR反应体系/分子标记Key words
AFLPs/ Camellia oleifera Abel./ genetic diversity/ PCR reaction system/ molecular markers分类
农业科技引用本文复制引用
金龙,刘冰,周明善,吴山忠,王勤,束庆龙,张良富,曹翠萍..油茶AFLP反应体系建立及其在遗传多样性研究上的应用[J].安徽农业大学学报,2012,39(4):497-501,5.基金项目
安徽省人才开发专项资金(2008Z023),安徽农业大学引进与稳定人才科研资助(yj2008-3)和安徽省自然科学基金(090411025)共同资助. （2008Z023）
