安徽农业大学学报2012，Vol.39Issue(4)：545-551,7.
黄连饮片中小檗碱的提取和重金属镉去除工艺的研究
Extraction of berberine and removal of cadmium from Coptis chinensis Franch.
摘要
Abstract
The organic solvents refluxing was used to extract berberine from Coptis chinensis Franch., and the extraction condition, including concentration of ethanol, extraction temperature, solid-liquid ratio were chosen as indices in the extraction process by the response surface method. In the same time, atomic absorption spectrophotometry was employed to test the contents of cadmium and berberine in Coptis chinensis Franch. after removal of cadmium with different treatments. The results showed that the best conditions for extraction berberine were using 53% ethanol, extraction at 62℃, with solid/solvent ratio of 1:11, extraction for 1 h and two times. The best treatment was using 3% ammonia for extracting six hours plus 3% NH4NO3 for six hours, and the content of cadmium is 0.037 6 mg·kg-1, less than 0.3 mg/kg, which meets the pharmacopoeia standards(2010 edition). The content of berberine is 8.0%.关键词
黄连饮片/小檗碱/响应面法/重金属/镉/去除Key words
Coptis chinensis Franch./ berberine/ response surface method/ heavy metal/ cadmium/ removal分类
医药卫生引用本文复制引用
高锐,杨小方,汪维云,童心,王勇..黄连饮片中小檗碱的提取和重金属镉去除工艺的研究[J].安徽农业大学学报,2012,39(4):545-551,7.基金项目
安徽省教育厅自然科学研究重点项目(KJ2011A123)资助. （KJ2011A123）
