安徽农业大学学报2012，Vol.39Issue(4)：571-575,5.
螺旋藻富铬及其含铬蛋白组份对α-葡萄糖苷酶抑制的研究
Effects of chromium (Ⅲ)-riched Spirulina platensis proteins on the activity inhibition of α-glucosidase
摘要
Abstract
In this paper, we studied the absorption degree of Cr(Ⅲ) in Spirulina platensis which cultivated in chromium (Ⅲ)-riched Zarrouk medium, by extracting proteins with ammonium sulfate precipitation, analyzing the content of Cr(Ⅲ) in protein components. And then we investigated the inhibition effect of chromium (Ⅲ)-riched Spirulina platensis proteins on alpha-glucosidase. The results showed that Spirulina platensis growed in culture medium added with 300 mg·L-1 CrCl3·6H2O, the total enriched Cr(Ⅲ) and its organic degree of Cr(Ⅲ) could reach to 3.30 mg·g-1 and 70.2%, correspondingly. When different saturated solution (25%, 50% and 75%) of ammonium sulfate were used to precipitate proteins, the proportion of Cr(Ⅲ) in protein components to total Cr(Ⅲ) were 2.1%, 16.8% and 7.0%, respectively. The abilities of different proteins combined with Cr (Ⅲ) were different. The three protein components had different inhibition activities on alpha-glucosidase. Among them, protein components precipitated by 50% saturation solution of ammonium sulfate revealed the best inhibition effect on alpha-glucosidase, with IC50 of 69.87 mg·mL-1.关键词
三价铬/螺旋藻蛋白/α-葡萄糖苷酶Key words
Chromium (Ⅲ)/ Spirulina platensis proteins/ α-glucosidase分类
农业科技引用本文复制引用
何颖敏,孙宜君,张娇,李博生..螺旋藻富铬及其含铬蛋白组份对α-葡萄糖苷酶抑制的研究[J].安徽农业大学学报,2012,39(4):571-575,5.基金项目
教育部重点研究项目(102023)和国家林业公益性行业科研专项(200704025)共同资助. （102023）
