安徽农业科学Issue(13)：7722-7725,7762,5.
利用棉花不同生育期的拮抗内生菌协同控制棉黄萎病研究
Antagonistic Endophytic Bacteria Isolated from Different Growth Stages of Cotton for the Control of Cotton Verticillium Wilt
摘要
Abstract
[Objective] This study aimed to investigate the combined control effects of endophytic bacteria at different growth stages against Verticittium dahliae and provide a new strategy for the biocontrol of other soil-borne diseases. [Method] Endophytic bacteria with high resistance against Verticillium dahliae were isolated from seedling, squaring and boll-setting cotton vascular, respectively. Their 16S rDNA sequences were detected for comparative analysis. Three biocontrol strains were selected and identified, whose colonization roles in cotton plants were explored. The control efficiency was determined with indoor and field experiments. [Result] According to the 16S rDNA sequence hn-mology, the three strains were identified as Paenlbaclllus poljmyxa YUPP-8, PaenibacUlus xylanilylicus YUPP-1 and Bacillus sublilis YUPP-2, respectively. Results of coionization assessment showed that three strains all could be successfully colonized in cotton vascular. But application amount had a positive effect on the number of colonized biocontrol bacteria in cotton. Strain YUPP-8 had the largest number of colonized biocontrol bacteria in seedling period. Strain YUPP-1 had the largest number of colonized biocontrol bacteria in squaring period, and strain YUPP-2 had the largest number of colonized biocontrol bacteria in boll-setting period. Indoor pot experiment showed that cotton plants in combined bioconlroi bacteria treatment group were not infected in flowing period, while Verticillium dahliae of cotton treated with single strain in seedling period were 6.7% (YUPP-8), 6.7% (YUPP-1) and 13.3% (YUPP-2); however, VertkiUium dahline. of the control reached 80%. Field experiment conducted during 2010-2011 showed that the combined application of three strains had better effect than separate application; specifically, Verticillium dahliae morbidity rate and disease index of cotton in boll-setting period with combined application of three strains in 2020 were 9. 4% and 6. 5 , respectively, while those in control group were 47. 5% and 32. 8 ; results in 2011 were similar to 2010, with higher disease severity. These results indicate that combined application of endophytic bacteria at different growth stages has great application potential in control of cotton Verticiltium dahliae. [Conclusion] This study preliminarily overcome the defects in the application of biocontrol bacteria and provided reference for the prevention and treatment of other soil-borne diseases.关键词
棉花黄萎病菌/多粘类芽孢杆菌/解木聚糖类芽孢杆菌/枯草芽孢杆菌/定殖/防效Key words
Verticillium dahliae/ Paenibacillus polymyxa/ PaenibaciUus xylanilytkus/ Bacillus subtilis/ Colonization/ Control efficiency分类
农业科技引用本文复制引用
周燚,杨廷宪,杨佩,王斌先,孙正祥..利用棉花不同生育期的拮抗内生菌协同控制棉黄萎病研究[J].安徽农业科学,2012,(13):7722-7725,7762,5.基金项目
农业部公益性行业(农业)科研专项(nyhyzx07-052) （农业）
农业微生物国家重点试验开放课题(AML200806) （AML200806）
湖北省教育厅重大项目(Z20091201) （Z20091201）
国家大学生创新性实验计划(091048922). （091048922）
