安徽农业科学2012，Vol.40Issue(14)：8031-8034,4.
应用正交试验法优选茎瘤芥高效遗传转化体系
Optimization of Genetic Transformation System of Tumorous Stem Mastard by Orthogonal Design
摘要
Abstract
[ Objective ] To establish the genetic transformation system, so us to lay foundation lor the genetic transformation of tumoroua stem mustard and other Brassica plants. [ Method] The effects of different time of precullure.cocullure,bacteria liquid concentration and dipping time on the transformation rate of lurnorous stem mustard were studied with orthogonal design. [ Result ] The best conditions for genetic transformation were 3 d preculture,4 d coculture, bacteria liquid concentration (OD600) 0.5 and 10 min dipping,then the Kan concentration was 30 mg/L. [Conclution] The optimum conditions for the transformation of tumorous stem mustard were selected, which greatly improved the transformation rale.关键词
茎瘤芥/正变法/遗传转化Key words
Tumorous Stem mustard/ Orthogonal design/ Genetic transformation分类
农业科技引用本文复制引用
梁婷,刘毅,蔡应繁,岳显可,江怀仲,何晓红..应用正交试验法优选茎瘤芥高效遗传转化体系[J].安徽农业科学,2012,40(14):8031-8034,4.基金项目
国家自然科学基金(31071461,30771311) （31071461,30771311）
重庆市自然科学基金(cste2007BB1328). （cste2007BB1328）
