安徽农业科学2012，Vol.40Issue(14)：8092-8093,8164,3.
骆驼刺根状茎中3种植物激素的分离与含量测定
Separation and Content Determination of Three Kinds of Endogenous Hormones in Rhizome of Alhagi sparsifolia
摘要
Abstract
[ Objective ] The research aimed to establish an effective extraction method of plant endogenous hormones and discuss the chromato-graphic conditions for determining three kinds of plant endogenous hormones in rhizome ramet of Alhagi sparsifolia simultaneously by reversed -phase high performance liquid chromatography( RP-HPLC). [ Method] Using RP-HPLC,plant endogenous hormones gibberellin (GA3), zeatin (ZR) and auxin indole-3 -acetic acid (IAA) were separated from rhizome ramet of A, sparsifolia and their contents were determined. [Result] Average recovery of GA, ,ZR and IAA were 98. 3% ,90.3% and 101.3% respectively,which indicated that this method could meet the demands of quantitative analysis and had little error. The conditions for separating three kinds of plant endogenous hormones from the rhizome ramet of A. sparsifolia by RP-HPLC were as follows:using methanol-acetic acid aqueous solution(with the volume ratio of 45:55) as the mobile phase,the flow-rate of 0.7 ml/min,lhe detection wavelength of 254 nm and the column temperature of 25 t. [ Conclusion] The research preliminarily established the high performance liquid chromalogruphy conditions for separating plant endogenous hormones from the rhizome of A. sparsifolia.关键词
骆驼刺/内源激素/反相高效液相色谱Key words
Alhagi sparsifolia/ Endogenous hormones/ RP-HPLC分类
农业科技引用本文复制引用
赵曼丽,杨迎春,杨世杰,袁祯燕..骆驼刺根状茎中3种植物激素的分离与含量测定[J].安徽农业科学,2012,40(14):8092-8093,8164,3.基金项目
新疆大学博士启动基金项目(7020428027) （7020428027）
国家大学生创新性实验计划项目(101075512). （101075512）
