安徽农业科学2012，Vol.40Issue(14)：8099-8101,3.
猪繁殖与呼吸道综合征病毒荧光定量PCR检测方法的研究
Establishment of Fluorescent Quantitative PCR Assay by TaqMan Probe Approach for PRRSV Detection
摘要
Abstract
The recombined plasmid pMD-ORF7 was constructed and used as standard positive template. And the FQ-PCR assay was constructed by quantitative concentration of serial 10 fold dilutions of pMD-ORF7 cDNA by optimizing circulation parameters. A standard curve was a-chieved. The result showed the sensitivity of this method was 1.0 ?10 0copy/ 祃. To detect three different samples by FQ-PCR repeatedly, the average circle threshold value was 0. 14, 0. 14 and 0.44, with the coefficient of variation of 0.01 % , 0.01 % and 0.02% , respectively. The FQ-PCR was proved to be with high sensitivity and repeatability. The detection of PRRSV in positive samples by both FQ-PCR and conventional RT-PCR was 100% , but the sensitivity of the former was 100 times higher than that of the latter.关键词
猪繁殖与呼吸综合征病毒/荧光定量PCR/Taqman探针Key words
Porcine reproductive and respiratory syndrome virus/ Fluorescent quantitative PCR/ Taqman probe分类
农业科技引用本文复制引用
郝贵增,付亮,张福良,李敬玺..猪繁殖与呼吸道综合征病毒荧光定量PCR检测方法的研究[J].安徽农业科学,2012,40(14):8099-8101,3.基金项目
河南省科技攻关重点项目(08050). （08050）
