安徽农业科学2012，Vol.40Issue(14)：8111-8112,8135,3.
致病疫霉拮抗芽孢杆菌的筛选及脂肽类物质分离的研究
Screening of Antagonistic Bacillus spp.Strains against Phytophthora infestans ( Mont.) de Bary and Isolation of Lipopeptide Substances
摘要
Abstract
[ Objective] The paper was to screen the antagonistic Bacillus spp. strains against Phytophthora infestans (Mont. ) de Bary and analyze its lipopeptide substances. [ Method] Using flat isolation and culture method,the bacteria that could produce Bacillus were isolated from rhizosphere soil,and carried out primary and secondary screening by confrontation culture. The lipopeplide substances of Y-3 strain with better antifungal effect were carried out HPLC analysis. [ Result] Nine strains of bacteria had certain antagonistic effects against P. infesians in primary screening, Y-1 and Y-3 strains had significant inhibition effect against the growth of P. infestans in secondary screening,with inhibition rale higher than 80% ; the crude extract of lipopeplide produced by Y-3 strain had better antagonistic effect against P. infestans ,with inhibition rate higher than 90%. HPLC analysis showed that the crude extract of lipopeptide mainly contained 8 components. [ Conclusion ] The lipnpeptide substances produced by Y-3 strain had significant inhibition effect, which had potential application value for biological control of potato late blight.关键词
致病疫霉/拮抗菌/芽孢杆菌/脂肽/生物防治Key words
Phytophthora infestans / Antagonistic bacteria/ Bacillus spp. / Lipopeptide/ Biological control分类
农业科技引用本文复制引用
吴艳清,蒋继志,梁廷银..致病疫霉拮抗芽孢杆菌的筛选及脂肽类物质分离的研究[J].安徽农业科学,2012,40(14):8111-8112,8135,3.基金项目
河北省自然科学基金资助项目(C2011201003). （C2011201003）
