安徽农业科学Issue(19)：9979-9981,3.
重组G蛋白基因工程菌高密度发酵研究
Study on the Conditions of High Cell Density Fermentation for the Engineering Bacteria Expressed Recombinant Protein SPG
摘要
Abstract
[Objective] To explore the high cell density fermentation for the engineering bacteria, so as to lay foundation for obtaining high-concentration and high-yield SPG. [Method] The bacteria were transferred to a fermenting cylinder for a fed-batch high-density culture, the fermentation factors of IPTG dose, oxygen, pH and culture methods were studied. [Result] Under high cell density fermentation, 80 - 150 g/L bacteria were obtained, per liter of fermentation liquid contained 1 g SPG. The fermentation conditions of SPG were 10% IPTC dose, I vvm ventilation, 30% -45% dissolved oxygen, 7.0 -7.2 pH, 0.2 mmol/L IPTC concentration, and 4 h time, after fermentation, the total protein in SPG accounted for above 20% of the bacterial protein. [Conclusion] High-concentration and high-yield SPG could be obtained through this high cell density cultivation.关键词
高密度发酵/基因工程菌/SPGKey words
High cell density culture/ Gene engineering bacteria/ Staphyloccocus aureus protein G分类
农业科技引用本文复制引用
张虎成,杨国伟,王晓杰,郭东月,李小瑞,王亚萍..重组G蛋白基因工程菌高密度发酵研究[J].安徽农业科学,2012,(19):9979-9981,3.基金项目
北京市科技计划面上项目(PXM2012_014306_000016). （PXM2012_014306_000016）
