重组G蛋白基因工程菌高密度发酵研究OA
Study on the Conditions of High Cell Density Fermentation for the Engineering Bacteria Expressed Recombinant Protein SPG
[目的]探索基因重组工程菌高密度发酵工艺,为得到高浓度和高产量的链球菌(Streotococcus)G蛋白(SPG)奠定基础.[方法]通过一级摇瓶、二级种子罐培养,以及将菌种转接到发酵罐并进行分批补料高密度培养,探讨了IPTG加入量等条件对发酵的影响,考察了接种量、氧气、pH、培养方式等发酵工艺.[结果]高密度发酵能得到至少80g/L的菌体,最高达到150 g/L,每升发酵液可得到1g的SPG.SPG高密度发酵的生产务件为:接种量10%,通气量1…查看全部>>
[Objective] To explore the high cell density fermentation for the engineering bacteria, so as to lay foundation for obtaining high-concentration and high-yield SPG. [Method] The bacteria were transferred to a fermenting cylinder for a fed-batch high-density culture, the fermentation factors of IPTG dose, oxygen, pH and culture methods were studied. [Result] Under high cell density fermentation, 80 - 150 g/L bacteria were obtained, per liter of fermentation l…查看全部>>
张虎成;杨国伟;王晓杰;郭东月;李小瑞;王亚萍
北京电子科技职业学院生物工程学院,北京100029北京电子科技职业学院生物工程学院,北京100029北京电子科技职业学院生物工程学院,北京100029北京电子科技职业学院生物工程学院,北京100029北京电子科技职业学院生物工程学院,北京100029北京电子科技职业学院生物工程学院,北京100029
农业科技
高密度发酵基因工程菌SPG
High cell density culture Gene engineering bacteria Staphyloccocus aureus protein G
《安徽农业科学》 2012 (19)
9979-9981,3
北京市科技计划面上项目(PXM2012_014306_000016).
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