安徽农业科学Issue(19)：10022-10023,2.
梭梭种子组织培养表面灭菌方法研究
Study on Surface Sterilization Method for Tissue Culture of Haloxylon ammodendron ( C.A.Mey.) Bunge Seeds
摘要
Abstract
[Objective] The aim was to select appropriate sterilization methods and explants for tissue culture of Haloxylon ammodendron (C. A. Mey. ). [Method] H. ammudendron ( C. A. Mey. ) seeds were used as experimental materials to investigate the sterilization results of different sterilization treatments and the effect of different seed sizes on the survival rate of aseptic seedlings. [Result] Sterilization of H. ammodendron (C. A. Mey. ) seeds achieved the best result by using 75% alcohol for 15 min and 0.1% mercuric chloride for 8 min. Seeds with diameter (c) >2.0 mm were used as explants and achieved relatively high survival rate of aseptic seedlings, which reached above 65%. [Conclusion] This study established a surface sterilization method for tissue culture of H. ammodendron (C. A. Mey. ) Bunge seeds.关键词
梭梭/灭菌方法/组织培养Key words
Haloxylon ammodendron (C. A. Mey.) Bunge/ Sterilization methods/ Tissue culture分类
农业科技引用本文复制引用
王栋,赵贵平..梭梭种子组织培养表面灭菌方法研究[J].安徽农业科学,2012,(19):10022-10023,2.基金项目
新疆生产建设兵团塔里木畜牧科技重点实验室开放课题(HS20901). （HS20901）
