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福建金线兰多倍体的诱导和倍性鉴定

禹华芳

安徽农业科学Issue(19):10079,10085,2.
安徽农业科学Issue(19):10079,10085,2.

福建金线兰多倍体的诱导和倍性鉴定

Induction and Ploidy Identification of the Polyploid of Anoectochilus fornosanus

禹华芳1

作者信息

  • 1. 湖南娄底职业技术学院农林工程系,湖南娄底417000
  • 折叠

摘要

Abstract

[Objective] To induce the polyploid of Anoectochilus fornosanus and identify its ploidy. [Method] The adventitious buds of Anoec tochilus fornosanus were respectively soaked and coated with colchicine to induce the polyploid, and the ploidy of the chromosome was observed under microscope. [Result] The ploidy was 35% when the adventitious buds were coated with 0.05% colchicine for 72 h; the ploidy identification showed that there were 40 diploid chromosomes and 80 tetraploid chromosomes. [Conclusion] The metnod can cultivate the polyploid of Anoeclochllm fomosannsand, improve its survival rate and per mu yield of its transplants.

关键词

福建金线兰(Anoectochilus fornosanus)/秋水仙素/多倍体诱导

Key words

Anoectochilus fornosanus/ Colchicine/ Polyploidy induction

分类

农业科技

引用本文复制引用

禹华芳..福建金线兰多倍体的诱导和倍性鉴定[J].安徽农业科学,2012,(19):10079,10085,2.

基金项目

湖南省教育厅科研基金项目(10C0285) (10C0285)

娄底科技局科研基金项目(2010kj005). (2010kj005)

安徽农业科学

0517-6611

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