安徽农业科学Issue(19):10079,10085,2.
福建金线兰多倍体的诱导和倍性鉴定
Induction and Ploidy Identification of the Polyploid of Anoectochilus fornosanus
摘要
Abstract
[Objective] To induce the polyploid of Anoectochilus fornosanus and identify its ploidy. [Method] The adventitious buds of Anoec tochilus fornosanus were respectively soaked and coated with colchicine to induce the polyploid, and the ploidy of the chromosome was observed under microscope. [Result] The ploidy was 35% when the adventitious buds were coated with 0.05% colchicine for 72 h; the ploidy identification showed that there were 40 diploid chromosomes and 80 tetraploid chromosomes. [Conclusion] The metnod can cultivate the polyploid of Anoeclochllm fomosannsand, improve its survival rate and per mu yield of its transplants.关键词
福建金线兰(Anoectochilus fornosanus)/秋水仙素/多倍体诱导Key words
Anoectochilus fornosanus/ Colchicine/ Polyploidy induction分类
农业科技引用本文复制引用
禹华芳..福建金线兰多倍体的诱导和倍性鉴定[J].安徽农业科学,2012,(19):10079,10085,2.基金项目
湖南省教育厅科研基金项目(10C0285) (10C0285)
娄底科技局科研基金项目(2010kj005). (2010kj005)