安徽农业科学Issue(19)：10080-10081,10174,3.
藏药桃儿七的快繁体系及其鬼臼毒素含量分析
Rapid Propagation System of Tibetan Medicine Sinopodophyllum hexandrum and Determination of Its Podophyllotoxin Content
摘要
Abstract
[Objective] To estahlish the tissue culture-transplanting system of Tibetan medicine S. hexandrum, and compare the podophyllo-toxin content in rhizomes between wild and cultured S. hexandrum. [Method] The seeds of 5. hexandrum were used as explants to induce callus, and the seedlings were acclimatized and transplanted, the podophyllotoxin content in rhizomes of wild and cultured S, hexandrum was determined with HPLC. [Result] The seeds on medium of MS + 1. 0 mg/L 2,4-D +0.5 mg/L GA3 got the best callus induction rate of 33. 33% ; the vermiculite was the best matrix for seedlings to survive, and the survival rate could reach up to more than 80% ; podophyllotoxin content in wild 5. hexandrum rhizomes was 1.01% , and that in cultured S. hexandrum rhizomes was 0.087% , [Conclusion] Through tissue culture, the podophyllotoxin can be produced without damaging wild resources, the study provides guidance for the introduction and cultivation of 5. hexandrum.关键词
桃儿七(Sinopodophyllum hexandrum)/愈伤组织/移栽/鬼臼毒素/HPIC/含量测定Key words
Sinopodophyllum hexandrum/ Callus/ Transplanting/ Podophyllotoxin/ HPLC/ Content determination分类
农业科技引用本文复制引用
叶耀辉,张文雪,张寿文,黄慧莲,马越兴..藏药桃儿七的快繁体系及其鬼臼毒素含量分析[J].安徽农业科学,2012,(19):10080-10081,10174,3.基金项目
江西省教育厅科学技术研究项目(No.09278) （No.09278）
2009-2010年度省“十一五”重点学科青年教师培养计划资助项目(院字[2010]35号). （院字[2010]35号）
