安徽农业科学Issue(19)：10153-10155,10250,4.
广西巴马小型猪PPARγ-2基因多态性与2型糖尿病易感性的相关性
Relationship between the Genetic Polymorphism of PPARγ-2 and the Susceptibility of Type 2 Diabetes Mellitus in Guangxi Bama
摘要
Abstract
[Objective] The research aimed to discuss the relationship between the genetic polymorphism of PPARγ-2 and the susceptibility of type 2 diabetes mellitus(T2DM)in Guangxi Bama mini-pig. [Method] 24 Guangxi Bama mini-pigs were fed with high-fat and high-sucrose diet and partial sequences of exon 2 of PPAR7-2 gene were amplified by PCR method. The contents of blood glucose and insulin in Guangxi Bama mini-pig under fasting conditions were determined and the glucose tolerance test( GTT) was also carried out. [Result] There was one SNP site (19813A→G)in partial sequence of exon 2 of the cloned PPARγ-2 gene. And two genotypes, AA(7 pigs) and AG(17 pigs)were detected. The fasting blood insulin content and 60 min blood glucose level in GTT in AG-genotype Guangxi Bama mini-pig were significantly higher than that of AA genolype( P < 0.05 ). The incidence of T2DM in AG-genotype Guangxi Bama mini-pig (71.4% ) was obviously higher than that of AA genotype (5.9% ). [Conclusion] The polymorphism of 19813A→G in exon 2 of PPARγ-2 gene was related with the susceptibility of T2DM in Guangxi Bama mini-pig.关键词
广西巴马小型猪/PPARγ-2基因/2型糖尿病Key words
Guangxi Bama mini-pig/ PPARγ-2 gene/ Type 2 diabetes mellitus(T2DM)分类
农业科技引用本文复制引用
梁家充,郭亚芬,陈江伟,杨柳,兰干球..广西巴马小型猪PPARγ-2基因多态性与2型糖尿病易感性的相关性[J].安徽农业科学,2012,(19):10153-10155,10250,4.基金项目
广西科技基础条件平台建设项目(11-31-09). （11-31-09）
