安徽农业科学Issue(19)：10170-10174,5.
猪裸卵体外胞质成熟研究
Researoh on the in vitro Cvtoplasmic Maturation of Denuded Porcine Oocytes
摘要
Abstract
[Objective] This study aimed to improve the in vitro maturation quality of denuded porcine oocytes and provide scientific basis for establishing a stable and efficient denuded oocyte culture system. [Method ] The first polar body extrusion rate, oocyle glutathione (GSH) content, positive rate of brilliant cresyl blue ( BCB) staining and development potential of activated oocytes or fertilized oocytes were employed as main indicators to investigate the effects of follicular mural granulosa cell ( MGC) coculture on cytoplasmic maturation of cumulus cell-removal oocyles (Denuded Oocyte, DO). [Result] According to in vitro maturation results, compared with DO group, the first polar body extrusion rate of porcine oocytes in DO + MGC group was not significantly different, but the nuclear maturation process was improved and was more similar to that in COC ( cumulus-oocyte complex ) group. Detection of GSH content in mature oocytes showed that there was no significant difference between DO + MGC group (optical density of 1 053.67 ) and COC group ( optical density of 1 426.00) or between DO + MGC group and COC +GC group (optical density of 1 541.00) , however, GSH content in mature oocytes of DO group (optical density of 724.67) was significantly lower than that of COC group and COC + GC group ( P < 0.05). Detection of glucose-6-phosphate dehydrogenase ( G6PDH) activity showed that there was no significant difference in BCB positive oocyte rate between DO + MGC group (88. 26%) and COC group (92.75% ) or between DO + MGC group and DO group (82.86% ), however, BCB positive oocyte rate of DO group was significantly lower than that of COC group (P < 0.05). Furthermore, the cleavage rate and blastocyst rate of activated mature oocytes derived from DO + MGC group (94.98% and 43.67%, respectively) were significantly higher than those from DO group (52.54% and 8. 97% , respectively) (P< 0.05), and were not significantly different compared with those from COC group (97. 11% and 38. 30% , respectively). In addition, the cleavage rate of fertilized oocytes derived from DO + MGC group (72.65% ) showed no significant difference compared with that from DO group (63.59% ) , but the blastocyst rate of DO + MGC group was significantly higher than that of DO group (9. 88% ) (P < 0.05). [Conclusion] MGC coculture can significantly improve the in vitro cytoplasmie maturation quality of denuded porcine oocytes and thereby enhance the subsequent developmental potential.关键词
裸卵/壁颗粒细胞/体外成熟/胞质成熟/猪Key words
Denuded oocytes/ Mural granuiosa cells/ in vitro maturation/ Cytoplasmic maturation/ Pig分类
农业科技引用本文复制引用
郇延军,解炳腾,朱江,孔庆然,刘忠华..猪裸卵体外胞质成熟研究[J].安徽农业科学,2012,(19):10170-10174,5.基金项目
国家自然科学基金(30871431) （30871431）
黑龙江省杰出青年基金(JC200905). （JC200905）
