Lrh-1基因在湖羊HPG轴中表达量变化研究OA
Study on the Expression Level Changes of Lrh-1 Gene in HPG Axis in Hu Sheep
[目的]揭示湖羊发情周期不同阶段下丘脑-垂体-性腺(HPG)轴组织中Lrh-1基因的表达变化规律.[方法]建立了湖羊Lrh-1基因的实时荧光定量(qRT-PCR)检测体系,测定其在发情前期、发情期、发情后期和间情期湖羊的下丘脑、垂体和卵巢间质组织中的表达量,分析Lrh-1基因在湖羊HpG轴的表达随发情周期变化的规律.[结果]建立的湖羊组织Lrh-1 mRNA检测体系扩增效率为92.44％,扩增效果良好.在湖羊的下丘脑、垂体和卵巢间质组织中均检测到…查看全部>>
[Objective] The aim of the study was to reveal the expression change laws of Lrh-1 gene in tissues of hypothalamus-pituitary-gonad (HPG) axis of Hu Sheep. [Method]Rhe quantitative real-time PCR detection system (qRT-PCR) was established to detect the expression of Lrh-1 gene in Hu Sheep. TV expression levels of Lrh-1 mRNA in tissues of hypothalamus, pituitary and gonad of Hu Sheep in proestrus, oestrum , diestrus and metoestrus were determined and the expres…查看全部>>
张伟;张尧;张君胜;王利红
江苏畜牧兽医职业技术学院,江苏泰州225300江苏畜牧兽医职业技术学院,江苏泰州225300江苏畜牧兽医职业技术学院,江苏泰州225300江苏畜牧兽医职业技术学院,江苏泰州225300
农业科技
湖羊Lrh-1qRT-PCR发情周期
Hu Sheep Lrh-1 qRT-PCR Estrous cycle
《安徽农业科学》 2012 (19)
10175-10177,3
江苏省自然科学基金项目(BK2010356)江苏牧医学院院级项目(HX200906).
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