安徽农业科学Issue(19)：10251-10252,10300,3.
土壤中纤维素降解真菌的筛选及酶活性分析
Screening and Analysing the Cellulose Degradation Enzyme Activity of Cellulose Degradation Fungi from Soil
摘要
Abstract
[Objective ] The research aimed to screen Fungi degradating wheat stem Cellulose and analyse the infect of C/N ratio on cellulose degradation enzyme activity. [Method] Using congo red appraisal medium method and filter paper degradation method screen Fungi from soil, characterizing the Fungi types by analysing fungi rDNA conservative domain sequence. The cellulose-enzyme activity of the two cellulose-decomposing strains cultured on different glucose and ( NH4)2SO4 content medium was analysised in this work. [Result]Two fungi strains exhibiting strong cellulolytic ability, named NY01 and NY02, were screened from soil in this study. Sequence analysing of fungi ITS conservative domain amplified from NY01 and NY02 DNA using the primer pair of ITS1 and ITS4 showed that NY01 and NY02 had 99% similarity in nu-cleotide sequence with Trichoderma and NY02 had 99% similarity in nucleotide sequence with Muco. Results show that the CMC enzyme activity and FPA enzyme activity was highest when the medium C/N ratio was 8:1. [Conclusion] The study provides the foundation for further exploration of the degradation of straw cellulose.关键词
土壤/纤维素降解/真菌/筛选/酶活性Key words
Soil/ Cellulose degradation/ Fungi/ Screening/ Enzyme activity分类
农业科技引用本文复制引用
陈吉宝,刘征,和丽丽,李贵娟,江千令,冯筱凡,陈亚楠..土壤中纤维素降解真菌的筛选及酶活性分析[J].安徽农业科学,2012,(19):10251-10252,10300,3.基金项目
河南省教育厅自然科学研究计划项目(2011B180038) （2011B180038）
南阳师范学院大学生实践教学活动创新项目(ZB-2012-34). （ZB-2012-34）
