安徽农业科学Issue(19)：10267-10268,10293,3.
不同浸提方法茯砖茶多酚和咖啡碱的HPLC测定
Determination of Polyphenols and Caffeine of Fuzhuan Tea Extracted with Different Methods by HPLC
摘要
Abstract
[Objective] To study the influence of extraction methods on the content of polyphenols and caffeine in Fuzhuan tea, and provide references for establishing the best extraction method. [Method] The influence of five extraction methods on the contents of eight polyphenols, including gallic acid (GA), ( - ) -gallocatechin ( GC) , ( - ) -epi gallocatechin (EGC), ( - ) -epigallocatechm gallate (EGCG), ( - ) -gal-locatechin gallate (GCG), ( - )-epicatechin (EC), ( - )-epicatechin gnllate (ECG) and ( - )-catechin gallate (CG) and caffeine(CAF) in Fuzhuan tea was studied by high performance liquid chromatography (HPLC). [Result] The eight phenolic compounds showed good linearity within the test range of 0. 000 6-0.300 0 mg/ml, and the polyphenols extracted with 70% aqueous methanol in water hath at 70 ℃ was comparatively more effective than that with distilled water in water bath at 100 ℃, and the content of caffeine extracted by distilled water in ultrasonic bath was the highest. [Conclusion] 70% methanol extraction was the best for the extraction of polyphenols and the distilled water ultrasonic extraction was much better for the extraction of caffeine.关键词
茯砖茶/高效液相色谱/多酚/咖啡碱/浸提方法Key words
Fuzhuan tea/ HPLC/ Polyphenols/ Caffeine/ Extraction method分类
农业科技引用本文复制引用
梁艳,黄亚亚,邓永亮,胡歆,陈桂梅,纪晓明,周兴长..不同浸提方法茯砖茶多酚和咖啡碱的HPLC测定[J].安徽农业科学,2012,(19):10267-10268,10293,3.基金项目
咸阳市2011年科技计划项目(2011K11-16). （2011K11-16）
