安徽农业科学2012，Vol.40Issue(21)：10861-10862,10872,3.
樱桃叶多糖的提取、鉴别及定量测定
Extraction, Identification and Content Determination of Polysaccharides from the Leaves of Prunus pseudocerasus Lindl
摘要
Abstract
[Objective] Tn sludy the extraction, qualitative identification and quantitative assay of polysaccharides from the leaves ol Prunus pseudocerasus Lindl. (PPP), so as to provide reference for its comprehensive application, [ Method ] Polysaccharides were extracted with the method of water exaction and alcohol precipitation after the leaves were defatted with 95% EtOH and petroleum ether. Then, the protein in PPP was removed by Sevag method. A series of experiments were used to identify the PPP, including Molish test ( o-naphthol lest) , anthrone-sulfuric acid method test, fehling test, fourier transform infrared speclrometry ( FT-1R), and iodine test. The content of PPP was determined by anthrone-sulphuric acid colrimetry method. [ Results] P. pseudocerasus polysacrharidea were grey white in color. The content of polysaccharides in P. pseudocerasus leaves was 29.7 mg/g. [Conclusion] The method is simple, sensitive, reproducible, and reliable.关键词
樱桃叶/多糖/提取/鉴定/硫酸-蒽酮法Key words
Prunus pseudocerasus Lindl. / Polysaccharides/ Extraction/ Identification/ Anthrone-sulfuric acid method分类
农业科技引用本文复制引用
刘德胜,颜玲,韩景田..樱桃叶多糖的提取、鉴别及定量测定[J].安徽农业科学,2012,40(21):10861-10862,10872,3.
