安徽农业科学2012，Vol.40Issue(22)：11147-11150,4.
GmSARK启动子驱动IPT基因在拟南芥中的表达研究
Heterologous Expression of Isopentenyl Transferase(Ipt) Gene Directed by Senescence Associated Receptor Protein Kinase(SARK) Promoter in Arabidopsis
摘要
Abstract
[Objective] This study aimed to investigate the expression of IPT gene directed by GmSARK promoter in Arabidopsis. [ Method] IPT gene and GmSARK promoter were respectively cloned to construct plant expression vector and transform Arabidopsis. T, transgenic plant lines were detected by PPT(phosphinothricin) herbicide selection and treated under drought and dark conditions for semi-quantitive RT-PCR analysis. [ Result] GmSARK and IPT fusion gene was successfully cloned and the plant expression vector p3301 -GmSAHK-IPT was constructed. RT-PCR analysis showed that the target gene was expressed in T1 transgenic plant lines at the mRNA level. [ Conclusion] This study laid the foundation for further investigating the roles of IPT gene directed by GmSARK promoter in plant stress resistance.关键词
IPT基因/GmSARK启动子/拟南芥Key words
IPT gene/ CmSARK promoter/ Arabidopsis分类
农业科技引用本文复制引用
霍巍巍,闫璇玲,孙晓文,李海燕..GmSARK启动子驱动IPT基因在拟南芥中的表达研究[J].安徽农业科学,2012,40(22):11147-11150,4.基金项目
国家转基因生物新品种培育重大专项(NO.2010ZX08010-002) （NO.2010ZX08010-002）
教育部新世纪优秀人才计划项目(NO.NCET-08-0693) （NO.NCET-08-0693）
吉林省优秀创新团队项目(NO.20111815) （NO.20111815）
长春市科技局项目(NO.09YJ24). （NO.09YJ24）
