安徽农业科学2012，Vol.40Issue(22)：11302-11304,11424,4.
玉米赤霉烯酮对猪睾丸间质细胞DNA损伤作用的彗星试验
Single Cell Gel Electrophoresis Assay of Porcine Leydig Cell DNA Damage Induced by Zearalenone
摘要
Abstract
[Objective] This study aimed to investigate the effect of zearalenone (ZEN) on DNA damage of porcine leydig cells. [Method] Porcine leydig cells cultured in vitro were collected to determine the median lethal dose (LD50 ) of ZEN with tetrazolium-based colorimetric assay (MTT assay). Comet assay was carried out to detect the DNA damage of porcine leydig cells exposed to at 0 (negative group) ,1,5, 10, 20, 40 μmol/L of ZEN. [Result] The percentage of cell tail was 16.67% , 34.00% , 40.67% , 52.00% and 64.67% after exposed to 0, 1 , 5, 10 and 20 μmol/L of ZEN, respectively; the differences between the percentages of cell tail in various experimental groups had extremely significant statistical significance compared with the negative group (P<0. 01) , showing a significant dose-effect relationship; Tail length in various groups was 57.60 ±4.78, 57.75 ±6.25, 78.97 ±5.83, 100.50 ±6. 94 and 146.83 ±12.31 μm, respectively; Tail DNA % in vari-ous groups was 21.29 ±2.25% , 22.24+2.43%, 31,21 ±6.27% , 37.45 ±4.33% and 60. 68 ±9.83%, respectively; Tail length and Tail DNA % in experimental groups with ZEN concentration above 5 μmol/L showed significant differences (P <0. 05) compared with the negative group, which showed an upward trend with the increase of ZEN concentration. [Conclusion ] ZEN has genotoxic effect on porcine leydig cells, which can cause DNA damage, with a significant dose-effect relationship.关键词
睾丸间质细胞/玉米赤霉烯酮/DNA损伤/彗星试验Key words
Leydig cells/ Zearalenone/ DNA damage/ Cornet assay (Single cell gel electrophoresis assay)分类
农业科技引用本文复制引用
甄建伟,刘青,顾建红,袁燕,刘学忠,王捍东,刘宗平,卞建春..玉米赤霉烯酮对猪睾丸间质细胞DNA损伤作用的彗星试验[J].安徽农业科学,2012,40(22):11302-11304,11424,4.基金项目
江苏省普通高校自然科学基金(08KJD230002)资助 （08KJD230002）
扬州大学科技创新培育基金资助. （）
