安徽农业科学2012，Vol.40Issue(22)：11314-11316,11319,4.
鲤鱼白细胞介素10(IL-10)基因cDNA克隆及序列分析
Cloning and Sequence Analysis of Interleukin 10 (IL-10) Full-length cDNA from Cyprinus carpio L.
摘要
Abstract
[Objective] This study aimed to obtain IL-10 (interleukin 10) full-length cDNA of common carp (Cyprinus carpio L. ) and conduct the sequence analysis. [Method] The differentially expressed cDNA fragment was obtained by DD-RTPCR (differential display RT-PCR) to get the full-length IL-10 cDNA. The cDNA library of peripheral blood leukocytes which were separated from common carp and stimulated with mitogen was screened by a probe labeled with DIG (digoxigenin). The IL-10 full-length cDNA was cloned from 0. 8 × 104 recombinant phages, and the sequence analysis and homology comparison, were carried out. [Result] Sequence analysis indicated that the IL-10 full-length cDNA of common carp was 1 117bplong, containing a 55 bp 5'-UTR, a 522 bp 3'-UTR, and a 540 bp open reading frame (ORF) encoding 179 amino acids. In addition, there were Lhree motifs for mRNA instability (ATTTA) in the 3'-untranslated region. The deduced protein sequence shared typical sequence features of the IL-10 family. Homology comparison indicated that the obtained sequence shared 89. 1% homology with the carp IL-10 gene from GenBank. [Conclusion] This study laid foundation for further study of the expression manner, functional characteristic and regulation mechanism of IL-10 in vivo and the interaction mechanism in the inflammatory reaction and immune response.关键词
鲤鱼/IL-10/克隆/序列分析Key words
Common carp/ Interleukin 10/ Cloning/ Sequence analysis分类
农业科技引用本文复制引用
冯祥汝,陈义龙,赵晓,王文东,张俊辉,杨振国,孙真,贾生美,卢强..鲤鱼白细胞介素10(IL-10)基因cDNA克隆及序列分析[J].安徽农业科学,2012,40(22):11314-11316,11319,4.基金项目
国家自然科学基金项目(30972277) （30972277）
吉林大学基本科研业务费项目(200903250). （200903250）
