安徽农业科学2012，Vol.40Issue(23)：11547-11548,11580,3.
一株产抗霉菌活性物质的海洋细菌鉴定
Identification of a Marine Bacteria Producing Bioactive Substances
楚振华 1钟响 1鞠宝1
作者信息
- 1. 烟台大学生命科学学院,山东烟台264000
- 折叠
摘要
Abstract
[Objective] The research aimed to study whether the microorganism in Styela clava could produce the active substance which possessed the antimicrobial properties. [Method] A bacterium N2 was screened from Styela clava in Yantai maritime space. The bacteriostasis function of N2 in the partial mold was explored by circle clear zone. The phylogenetic analysis results basically consisted with morphologic observation and analysis results. Based on the phenotypic properties of morphology, physiology and biochemical, the identification of bacteria was made almost thoroughly and systemically. Taxonomical status of this bacterium was further demonstrated by sequencing 16S rRNA gene of strain N2, comparing with those of related strains and constructing a phylogenetic tree using neighbour-joining method. [Result] N2 had strong antibacterial activity to the growth of mold. The bacterium was gram-negative which was rod-shaped and had flagellum. The microflora was opaque ivory, spore growed at secund strain N2 was similar to Bacillus in the most of the phenotypes. Strain N2 was most relative to Bacillus subtilis, and the homology of 16S rRNA gene between them was 99%. [Conclusion] Strain N2 was identified as Bacillus subtilis. It can be used in intensive aquaculture as biologicals using to anti-tumor and prevent disease of plants.关键词
柄海鞘/海洋细菌/16S rDNA/分子鉴定/枯草芽孢杆菌Key words
Styela clava/Marine bacteria/ 16S rDNA/ Molecular biology identification/Bacillus subtilis分类
农业科技引用本文复制引用
楚振华,钟响,鞠宝..一株产抗霉菌活性物质的海洋细菌鉴定[J].安徽农业科学,2012,40(23):11547-11548,11580,3.
