安徽农业科学2012，Vol.40Issue(23)：11558-11559,11561,3.
北柴胡药材的HPLC指纹图谱研究
HPLC Fingerprint Chromatogram of Bupleurum chinense DC.Medicinal Materials
王一飞 1王广基 2佟玲 1张瀛 2朱永宏2
作者信息
- 1. 中国药科大学药物代谢动力学重点实验室,江苏南京210009
- 2. 天津天士力集团研究院,天津300410
- 折叠
摘要
Abstract
[Objective] To establish HPLC fingerprint for Bupleurum chinense DC. . [Method] The Bupleurum chinense DC. samples from nine regions were gradient eluted with an Agilent Zorbax SB-C18 column(4.6 mm ×250 mm,5.0 μm)at 25 ℃ and a mobile phase consisting of 0.05% phosphoric acetonitrile (A) and water at a flow rate of 1.0 ml/min and at the detection wavelength of 220 nm, their fingerprints were analyzed. The similarity of the fingerprints was analyzed according to the software in Similarity Evaluation of the Fingerprints of Traditional Chinese Medicine ( edition A of 2004) published by Chinese Pharmacopoeia Commission. [ Result ] 11 peaks were identified as common peaks. Each peak showed good separating degree, the RSDs of the relative retention rate of common peaks were below 1.0% with the similarity of the fingerprints of nine Bupleurum chinense DC. samples above 90% . [Conclusion] With saikoside a as internal reference peak, a HPLC fingerprint method was established for Bupleurum chinense DC. , the method is table and has good reproducibility, thus it can be used for the quality control of Bupleurum chinense DC. .关键词
高效液相/柴胡/指纹图谱/柴胡皂苷aKey words
HPLC/Bupleurum chinense DC. / Fingerprint/ Saikosaponin a分类
农业科技引用本文复制引用
王一飞,王广基,佟玲,张瀛,朱永宏..北柴胡药材的HPLC指纹图谱研究[J].安徽农业科学,2012,40(23):11558-11559,11561,3.
