安徽农业科学2012，Vol.40Issue(23)：11595-11597,3.
一株产纤维素酶真菌Mucor amphibiorum RSC1的分离鉴定及其产酶培养基的初步优化
Isolation and Identification of a Strain of Cellulase-producing Fungus Mucor amphibiorum RSC1 and Preliminary Optimization of Its Medium
摘要
Abstract
[ Objective] To isolate and identify a strain of cellulase-producing fungus,so as to provide experimental basis for improving its species diversity. [ Method] The cellulase-producing fungus was firstly screened by Congo red method and then rescreened by flask fermentation from haystack, the fermentation culture medium was optimized by single factor test, and the morphology and 18S rRNA of this fungus were identified. [Result] A strain of cellulase-producing fungus RSC1 was isolated,as indicated by the optimization results of fermentation medium,the activity of produced cellulase was the highest on the medium containing 1.3% CMC-Na,0. 1% yeast extract and 0.1% MgSO4. As shown by the morphology and molecular biology of this fungus,the similarity between RSC1 and Mucor amphibiorum was as high as 97% ,thus the fungus was named as Mucor amphibiorum RSC1. [Conclusion] The study identified the isolated fungus to be Mucor amphibiorum RSC1,which laid foundation for improving the species diversity of cellulase-producing fungus.关键词
纤维素酶/产纤维素酶真菌的分离/Mucor amphibiorum RSC1Key words
Cellulase/ Isolation of cellulase-producing fungus/Mucor amphibiorum RSC1分类
农业科技引用本文复制引用
王舒,李汉骏,查美玲,付鸣佳..一株产纤维素酶真菌Mucor amphibiorum RSC1的分离鉴定及其产酶培养基的初步优化[J].安徽农业科学,2012,40(23):11595-11597,3.基金项目
国家自然科学基金(31060004) （31060004）
江西省自然科学基金(2010GZN0115) （2010GZN0115）
江西省教育厅科技项目(GJJ11387). （GJJ11387）
