安徽农业科学2012，Vol.40Issue(23)：11616-11618,3.
四川泸州桂圆的RAPD分析
Random Amplification Polymorphic DNA (RAPD) Analysis of Luzhou (Sichuan) Dimocarpus longgana Lour
梅志强 1李春红 2龚舒 1刘晓燕 1于海清1
作者信息
- 1. 泸州医学院医学基础研究中心,四川泸州646000
- 2. 泸州医学院药物与功能性食品研究中心,四川泸州646000
- 折叠
摘要
Abstract
[ Objective ] To identity Dimocarpus longgana Lour, samples from Sichuan Province (Luzhou city) and Guangxi Province, and detect their genetic relationships. [Method] With the D. longgana from Huangyi, Haichao, Chaohe and Zhangba of Luzhou and Guangxi Province as experimental materials, the genomic DNA were extracted from their leaves by CTAB method. The improved Random Amplification Polymorphic DNA (RAPD) technique was adopted to select twenty random primers with good reproducibility for further RAPD analysis of five D. longgana samples by prolonging the ramp time from annealing to extension. [ Result ] The results showed that the resolution and production can be increased by reducing ramp parameter. 124 clear and reproducible fragments were yielded, 58% of which, about 72 bands were polymorphic. The RAPD specific bands were detected among five D. longan samples, and the lowering of ramp parameters could improve the resolution and yield of PCR products. According to the genetic dendrogram of the 124 bands, the similarity index values ranged from 0.59 to 0. 80. As shown in UPG-MA cluster analysis result, D. longan of Guangxi was at the base of the dendrogram, while four cultivars from Luzhou formed sister clades. Haichao and Zhangba D. longan were clustered together with a 0. 80 similarity, followed by Chaohe and Huangyi clade from Luzhou with a 0.78 similarity. [Conclusion] Therefore, Luzhou D. longan belongs to two gene pools. The improved RAPD technique is effective in identifying D. longan cultivars and analyzing their genetic relationships.关键词
RAPD/ramp/桂圆/泸州Key words
RAPD/ ramp/Dimocarpus longgana Lour. / Luzhou分类
农业科技引用本文复制引用
梅志强,李春红,龚舒,刘晓燕,于海清..四川泸州桂圆的RAPD分析[J].安徽农业科学,2012,40(23):11616-11618,3.
