安徽医科大学学报2012，Vol.47Issue(10)：1174-1178,5.
维生素D类似物EB1089对肝癌细胞增殖凋亡侵袭的影响
Effects of vitamin D analogues EB1089 on proliferation,apoptosis and invasion of heptocellular cells
摘要
Abstract
Objective To invesligate the effects of vitamin D analogue EB1089 on the proliforation , apoptosis , in-vasion and migration of heptocellular cells. Methods MHCC97H heptocellular cell lines were treated with EB1089 of different concentration in different period of time. The conditions of cell proliferation was analyzed by MTT methods. The cell apoptosis was analyzed by flow cytometer. The conditions of cell migration and invasion were analyzed by wound healing assays as well as transwell assays. The expression of ERα,NF-κB p65 and MMP-9 mRNA in cells were tested by RT-PCR methods. Results EB1089 could inhibit the proliferation, invasion and induce apoptosis of heptocellular cell lines. Meanwhile, EB1089 increased the expression of ERα mRNA and decreased the expression of NF-ΚB p65 and MMP-9 mRNA. Conclusion EB1089 may inhibit the proliferation, invasion and induce apoptosis of heptocellular cell lines via ERα关键词
维生素D/肝癌/雌激素受体αKey words
vitamin D/ hepatocellular carcinoma/ estrogen receptor a分类
医药卫生引用本文复制引用
盛茂林,许戈良,荚卫东,任维华,刘文斌,张传海,周腾..维生素D类似物EB1089对肝癌细胞增殖凋亡侵袭的影响[J].安徽医科大学学报,2012,47(10):1174-1178,5.基金项目
国家自然科学基金(编号:30972893) （编号:30972893）
安徽省"115"产业创新团队项目 （）
