安徽医科大学学报2012，Vol.47Issue(10)：1178-1181,4.
绞股蓝总皂苷含药血清对光老化HaCaT细胞NF-κB通路的影响
Effect of gypenosides-containing serum on the NF-κB pathway in photoaging HaCaT cell
摘要
Abstract
Objective To study the effect of gypenosides-containing serum on the expression of tumor necrosis fac-tor-alpha( TNF-α )and nuclear factor kappa B( NF -κB ) protein in photoaging HaCaT cell. Methods The photoag-ing HaCaT cell model was established by UVB radiation and intervention was made with different concentrations of gypenosides-containing rat serum, 12 hours later, the expression of TNF-α and NF -κB protein in each group cells were measured by ELISA and Western blot. Results Compared with the control group, the expression of TNF-α of cell culture supernatant and NF -κB protein in UV model group cells increased significantly. Compared with the UV model group, the expression of TNF-α of cell culture supernatant and NF -κB protein in low, middle and high gypenosides-containing serum group cells decreased significantly. Conclusion Inhibiting the NF-κB signal pathway is one of the mechanisms of gypenosides-containing serum in preventing skin photoaging probably.关键词
光老化/绞股蓝总皂苷/人永生化皮肤角质形成细胞株/中波紫外线/肿瘤坏死因α/核因子-κBKey words
photoaing/ gypenosides/ HaCaT/ UVB/ TNF-α/ NF-ΚB分类
医药卫生引用本文复制引用
陶冶,张小卿,马贤德,吴景东..绞股蓝总皂苷含药血清对光老化HaCaT细胞NF-κB通路的影响[J].安徽医科大学学报,2012,47(10):1178-1181,4.基金项目
辽宁省自然科学基金(编号:20102149) （编号:20102149）
