北华大学学报：自然科学版2012，Vol.13Issue(2)：167-172,6.
葫芦巴总皂苷对心肌成纤维细胞转分化的影响及机制的实验研究
Experimental Study on Effects of TFGs on Cardiac Fibroblast Transdifferentiation and Its Mechanisms
摘要
Abstract
Objective To investigate the intervention of Trigonella foenum greacum. L Saponin （TFGs） on urotensin Ⅱ-induced cardiac fibroblasts （CFs）transdifferentiation cultured rats and its related molecular mechanism. Method Cultured CFs were randomly divided into control group, U Ⅱ stimulation group and TFGs intervention group. CFs of the control group were not given any stimulation; CFs of U Ⅱ stimulation group werecultured with 10 -8 mol/L U Ⅱ and the cultures were terminated at 12,14 and 48 hours ; CFs of TFGs intervention group were added U Ⅱ as a stimulus ,whose final concentrations were 0,25,50,100 and 200 mg/L respectively. The expression of a-SMA was observed with immunofluorescence and immunohistochemistry. The expression of connective tissue growth factor （CTGF） was assessed with RT-PCR and Western blotting. Results The results from immunofluorescence showed that there was a little expression of a-SMA in the cytoplasm of cells of the control group after 24-hour culture, but a-SMA expression after the U Ⅱstimulation increased significantly. The results from immunohistochemistry showed that a-SMA expression increased gradually with the intervention of U H at the different times （ 12,24 and 48 hours）. The CFs transdifferentiation induced by U Ⅱ could be inhibited by the culture solution containing TFGs, and the inhibition showed a dose-dependent and time-dependent manner. Compared with those in control group, the expressions of CTGF gene and its protein in U Ⅱ stimulation group increased significantly （ P 〈 0.01 ）, but the regulation of the expressions induced by U Ⅱ could be inhibited by TFGs （P 〈 0.01 ）. Conclusion The transdifferentiation of cardiac interstitial fibroblasts into a-SMA ＋ myofibroblasts of rats may be induced by U Ⅱin vitro. The transdifferentiation may be partially related to the increased CTGF expression. TFGs can effectively inhibit the transdifferentiation of CFs and reduce U Ⅱ -induced overexpression of CTGF. This study should further clarify the role of U Ⅱ in myocardial fibrosis, and also provide an experimental basis for the application of TFGs in the clinical prevention and treatment of myocardial fibrosis.关键词
葫芦巴总皂苷/尾加压素Ⅱ/成纤维细胞/转分化/结缔组织生长因子Key words
Trigonella foenum greacum. L Saponin/Urotensin Ⅱ/fibroblasts/transdifferentiation/connective tissue growth factor分类
医药卫生引用本文复制引用
曲萌,崔继春,董志恒..葫芦巴总皂苷对心肌成纤维细胞转分化的影响及机制的实验研究[J].北华大学学报：自然科学版,2012,13(2):167-172,6.基金项目
基金项目：吉林省科技发展计划项目 （）
北华大学校管课题（2008140）. （）
