北京林业大学学报2012，Vol.34Issue(4)：52-57,6.
利用ISSR分析技术鉴定贵州枇杷新种质的研究
Identification of new germplasms of Guizhou loquat using ISSR markers
摘要
Abstract
The fingerprinting and genetic relationship of 18 loquat（Eriobotrya japonica） cultivars,as well as the newly-bred lines were characterized by ISSR markers to explore the appraisal method of loquat germplasms.Fifteen selected ISSR primers were used for PCR amplification.The results showed that,a total of 172 bands with distinct and good repeatability were scored,among which 157 were polymorphic,and polymorphic rate was up to 91.3%.All of the tested cultivars （lines） might be highly distinguished by ISSR markers generated by combinations of primers 825,834 and 848.The genetic relationships of the tested genotypes were unraveled using UPGMA method based on the coefficient computed with ISSR markers,and two groups were obtained from dendrogram taking 0.60 as a threshold.Therefore,ISSR markers may be effectively employed to genotype the loquat germplasms,as well as to elucidate their genetic relationships.关键词
枇杷/栽培品种/ISSR/指纹图谱/亲缘关系Key words
loquat/cultivar/ISSR marker/finger-printing/genetic relationship分类
农业科技引用本文复制引用
范付华,杨勇胜,李庆宏,邓勇,文晓鹏..利用ISSR分析技术鉴定贵州枇杷新种质的研究[J].北京林业大学学报,2012,34(4):52-57,6.基金项目
基金项目：贵阳市农业攻关项目（[2009]筑科农合同字第2-003号）、贵州大学研究生创新基金项目（校研农2011011）. （）
