北京林业大学学报2012，Vol.34Issue(4)：58-61,4.
光皮桦茎叶cDNA文库构建及部分EST序列SSR分析
Library construction of cDNA and SSR analysis of partial ESTs for stem and leaf of Betula luminifera
摘要
Abstract
A cDNA library of stem and leaf from Betula luminifera was constructed.The primary titer of cDNA library was about 1.5×106 pfu/mL,its recombinant efficiency reached 97.3%,and the size of insert DNA fragments ranged from 0.5 to 3.0 kb,with an average of 1.3 kb.The results indicated that it was a higher-quality cDNA library,and could be used in gene cloning and gene expression profile analysis.Distribution and frequency of SSRs were analyzed in 224 non-redundant ESTs from B.luminifera cDNA library,using online searching software.The results showed that 60 SSRs distributed in 47 EST sequences,accounting for 26.80% of all ESTs.Dinucleotide would be the major repeat types,accounting for 70.00% of the total number of acquired SSRs.The tri-nucleotide and tetra-nucleotide repeats accounted for 28.30% and 1.70% respectively.This research might lay the foundation for designing the targeted EST-SSR primers and genetic diversity analysis by mining the information of EST-SSR loci in B.luminifera EST sequence data.关键词
光皮桦/cDNA文库/SSR分析Key words
Betula luminifera/cDNA library/SSR analysis分类
农业科技引用本文复制引用
张敏,黄华宏,林二培,周厚君,王亚辉,童再康..光皮桦茎叶cDNA文库构建及部分EST序列SSR分析[J].北京林业大学学报,2012,34(4):58-61,4.基金项目
基金项目：浙江省重大科技专项重点项目（2008C02004-1）、浙江省林业科技重点项目（2006SY01）. （）
