草业科学2012，Vol.29Issue(8)：1224-1228,5.
农杆菌介导的smt1富硒基因转化紫花苜蓿研究
Transformation of alfalfa with smtl gene mediated by agrobacterium
摘要
Abstract
Selenium is an essential micronutrient for normal functions of alfalfa （Medicago sativa）. The scarcity of selenium would depress growth of alfalfa and decrease nutritional values of alfalfa hay, which is one of the key problems of low quantity and quality of alfalfa hay in China. Development of transgenic plants with the selenium-tolerant genes is a potential pathway to improve selenium level in alfalfa. The gene smtl cloned from Astragalus bisulcatus was introduced into alfalfa ＇ Zhongmu No. 1 ＇ plants mediated by agrobacterium. 32 transgenic plants were obtained after the screening of 3 mg · L-1 Hygromycin. 18 out of 32 were amplified bands consistent with smtl gene size by PCR and 2 were positive in the gene ex- pression identified by RT-PCR. Results indicated that the smtl gene had been transformed successfully and expressed normally in alfalfa.关键词
smt1基因/紫花苜蓿/硒Key words
smtl gene/alfalfal selenium分类
农业科技引用本文复制引用
李晶,任卫波,郭慧琴,王茅雁,刘雅学..农杆菌介导的smt1富硒基因转化紫花苜蓿研究[J].草业科学,2012,29(8):1224-1228,5.基金项目
内蒙古自然基金资助项目 （）
中央级公益性科研院所基本科研业务费专项资金 （）
