重庆理工大学学报：自然科学2012，Vol.26Issue(4)：40-44,5.
重组人角质细胞生长因子-Ⅰ的聚乙二醇修饰及体外活性
Study on PEGylation of Recombinant Keratinocyte Growth Factor-I and Activity in vitro
摘要
Abstract
The research studied the PEGylation of mPEG-maleimide （mPEG-Mal） of recombinant ke- ratinocyte growth factor-I mutant deleted on N-terminus （ △N23KGF-I） expressed in E. coli, and i- dentified the modified site and preliminary biological activity in vitro. Firstly, get the Pegylated AN23KGF-I; Then, identify all the segments of the peptide by the reduced and non-reduced peptide mass mapping （PMM）. Next, identify the modified site of △23KGF-I-mPEG-Mal-20K by the reduced PMM. Lastly, measure the activity of △N23KGF- I international standard product, △N23KGF-I, △N23KGF-I-mPEG-Mal-20K with BAF-3 cell line. And mPEG-Mal-20K is modified uniformly on Cys40 when urea exists. In contrast to △N23KGF-I international standard product, the activity of △N23KGF-I keeps about 116%, and the △N23KGF-I-m PEG-Mal-20K keeps about 36%.关键词
重组人角质细胞生长因子/质量肽图/聚乙二醇修饰Key words
keratinoeyte growth factor/peptide mass mapping(PPM)/PEGylation分类
生物学引用本文复制引用
冯一建,陈勇,张增涛,黄程,李红亮,胡春兰,范开..重组人角质细胞生长因子-Ⅰ的聚乙二醇修饰及体外活性[J].重庆理工大学学报：自然科学,2012,26(4):40-44,5.基金项目
国家科技重大专项资助项目 （）
重庆市自然科学基金资助项目 （）
