重庆医学2012，Vol.41Issue(27)：2810-2811,2815,封3,4.DOI:10.3969/j.issn.1671-8348.2012.27.007
维吾尔族妇女子宫颈癌和CIN中miRNA-101的表达及其与HPV的关系
Expression of miRNA-101 and its relationship with HPV in cervical carcinoma tissue and CIN of Uighur women
摘要
Abstract
Objective To investigate the expression of miRNA-101 in cervical cancer and cervical intraepithelial neoplasia(CIN) class Ⅰ- Ⅲ in Uighur women and to analyze its relation with human papillomavirus(HPV) infection. Methods The expression of miRNA-101 was detected using the method of locked nucleic acid(LNA)-modified oligonucleotide in situ hybridization(ISH) with the formalin-fixed paraffin-embedded(FFPE) specimens from cervical cancer, chronic cervicitis and CIN class Ⅰ ,Ⅱ , Ⅲ. Then the correlation between the expression of miRNA-101 and HPV infection in cervical cancer was analyzed. Results The expressin rates of miRNA-101 in chronic cervicitis, CIN Ⅰ , Ⅱ , Ⅲ and cervical cancer were 80. 00%, 73. 33%, 46. 67% ,26. 67% and 10. 00 % respectively with significant difference among the five groups. miRNA-101 in cervical cancer was correlated with HPV infection(R = - 0. 767,P = 0. 000). Conclusion miRNA-101 involves in the development and progression of cervical squamous cell cancer and may be related to the HPV infection. miRNA-101 may be used as the molecular marker of screening and early diagnosis in cervical cancer.关键词
宫颈上皮内瘤样病变/子宫颈/肿瘤/微RNAs人乳头瘤病毒Key words
cervical intraepithelial neoplasia/cervix uteri/neoplasms/microRNAs/human papillomavirus引用本文复制引用
叶静,林晨,贠江力,古力娜尔·库尔班..维吾尔族妇女子宫颈癌和CIN中miRNA-101的表达及其与HPV的关系[J].重庆医学,2012,41(27):2810-2811,2815,封3,4.基金项目
新疆维吾尔自治区教育厅高校科研计划重点项目(XJEDU2009I23). （XJEDU2009I23）
