重庆医学2012，Vol.41Issue(27)：2852-2853,2856,3.DOI:10.3969/j.issn.1671-8348.2012.27.023
甘草酸二铵对人肝癌细胞SMMC-7721增殖和p53表达的影响
Effect of diammonium glycyrrhizinate on proliferation in liver cancer cell SMMC-7721 and p53 expression
摘要
Abstract
Objective To investigate the inhibiting effect of diammonium glycyrrhizinate(DG) on proliferation of liver cancer cell SMMC-7721 in vitro and its effect on p53 expression. Methods Two groups were set up:the study group(DG intervention) and the control group without DG. MTT assay was used to measure the cell growth and the inhibiting effect of DG on SMMC-7721 cells. P53 mRNA was quantified by real-time fluorescence quantitative PCR, followed by Western blot for detecting p53 protein expression. Results The survival rate of SMMC-7721 cell was decreased by DG(0. 50,1. 00,2. 00,4. 00,8. 00,16. 00 mg/L) in a concentration-dependent manner,and the IC50 value for 48 h was(4. 31 ± 0. 21) mg/L. The inhibition rate was respectively 44. 52%, 66. 78% and 91. 24% in 4. 00,8. 00,16. 00 mg/L treatment groups. The gene and protein expressions of p53 were obviously up-regulated after exposure to DG in 2. 00,4. 00,8. 00 mg/L treatment groups. Conclusion DG inhibits the growth of liver cancer cell SMMC-7721 obviously. And its mechanism may be related to up-regulate p53 expression.关键词
甘草酸/肝肿瘤,实验性/基因,p53/细胞增殖Key words
glycyrrhizic acid/liver neoplasms,experimental/genes,p53 /cell proliferation引用本文复制引用
张剑锋,李浩,李超乾,赵春菱,唐华民,严若谷..甘草酸二铵对人肝癌细胞SMMC-7721增殖和p53表达的影响[J].重庆医学,2012,41(27):2852-2853,2856,3.基金项目
广西教育厅科研课题资助项目(201012MS057) （201012MS057）
广西医疗卫生重点科研课题资助项目(重2011092). （重2011092）
