动物营养学报2012，Vol.24Issue(4)：712-721,10.DOI:10.3969/j.issn.1006-267x.2012.04.016
鲈鱼视黄酸受体α和视黄酸受体γ cDNA克隆和基因表达分析
Retinoic Acid Receptor α and Retinoic Acid Receptor γ in Lateolabrax japonicas : cDNA Cloning and Gene Expression Analysis
摘要
Abstract
This study was conducted to investigate structures of retinoic acid receptor α (RARα) and retinoic acid receptor γ (RARγ) and gene expression profiles in tissues. Reverse transcription PCR (RT-PCR) and rapid amplification of cDNA ends (RACE) methods were applied for the cloning of full-length cDNA coding for RARa and RAR7. Gene expressions of RARa and RARy in various tissues, such as liver, muscles, heart, eyes, intestine, kidney, fat, spleen, gill and brain, were determined. The results showed as follows; 1) full-length cDNA coding for RARa was 2 094 bp, which included a 124 bp of 5'- untranslated region (UTR) , a 608 bp 3'-UTR and a 1 362 bp open reading frame (ORF) , the predicted RARa consisted of 453 amino acids with a molecular weight of 56. 20 ku, and the theoretical isoelectric point was 8. 20. 2) Full-length cDNA coding for RAR7 was 1 671 bp consisting of a 36 bp 5'-UTR, a 129 bp 3'-UTR and a 1 506 bp ORF, 501 a-mino acids with a theoretical isoelectric point of 4. 96 and a molecular weight of 56. 20 ku was coded. 3) Both RARα and RAR7 had typical nuclear receptor structure, and they shared 63. 1 % identity in their amino acid sequences. Lateolabrax japonicus RARa and RARγ shared high identity (96. 9% and 95. 2% , respectively) with those of Takifugu rubripes. 4) Lateolabrax japonicus RARa and RARy gene expressions were detected in all the tested tissues, the higher gene expressions of RARa were in eyes, gill and intestine, the lower values were in heart and brain, and the higher gene expressions of RARy were in gill and spleen. In conclusion, full-length cDNA of RARa and RARγ in Lateolabrax japonicas are successfully cloned in this study, typical nuclear receptor structure is contained both in RARa and RAR7, and they show ubiquitous tissue distribution characteristics.关键词
维生素A/视黄酸受体/克隆/表达/鲈鱼Key words
vitamin A/ retinoic acid receptor/ clone/ expression/ Lateolabrax japonicus分类
农业科技引用本文复制引用
钱云霞,韩柳,童彩环,郑伟贤..鲈鱼视黄酸受体α和视黄酸受体γ cDNA克隆和基因表达分析[J].动物营养学报,2012,24(4):712-721,10.基金项目
国家自然科学基金资助项目(30671608) （30671608）
