动物营养学报2012，Vol.24Issue(5)：822-827,6.DOI:10.3969/j.issn.1006-267x.2012.05.005
奶牛静脉血内毒素浓度及其与产奶量的相关性
Endotoxin Concentration in Venous Blood and Its Correlation with Milk Yield of Dairy Cows
摘要
Abstract
In order to investigate the range of endotoxin concentration in venous blood of dairy cows and the correlation between endotoxin concentration and milk yield under main feeding modes at present in China, the feasibility of quantification of endotoxin concentration using the method of limulus amebocyte lysate chromoge-nic endotoxin was validated, and endotoxin concentration in venous blood of 89 dairy cows from three dairy farms was analyzed. They were farm 1 in Anhui province [60 cows; ratio of concentration to forage (C:F) was 45:55] , a farm in Jiangsu province (15 cows; ratios of concentration to forage were 20:80, 45:55 and 50:50, respectively, and those were set according to milk yield) ,and farm 2 in Anhui province (14 cows; ratio of concentration to forage was 70:30). Correlation between endotoxin concentration and milk yield was also analyzed. The results showed as follows: the limulus amebocyte lysate chromogenic endotoxin assay can be used for quantification of endotoxin concentration in venous blood of dairy cows, and endotoxin concentration of cows ranged from 0 to 1. 50 EU/mL. The correlation between endotoxin concentration and milk yield was not significant (P > 0. 05) , while correlation coefficient of dairy cows in Anhui farm 2 (0. 129 3) was higher than that of dairy cows in Anhui farm 1(0. 020 5) and Jiangsu farm (0. 037 6). The results indicate that limulus amebocyte lysate chromogenic endotoxin assay can be used for quantification of endotoxin concentration in blood of dairy cows; under main feeding modes at present in China, different concentrations of endotoxin are observed in blood of dairy cows, and there is no correlation between endotoxin concentration and milk yield.关键词
奶牛/内毒素/产奶量/相关性Key words
dairy cows/ endotoxin/ milk yield/ correlation分类
农业科技引用本文复制引用
张瑞阳,王东升,朱伟云,毛胜勇..奶牛静脉血内毒素浓度及其与产奶量的相关性[J].动物营养学报,2012,24(5):822-827,6.基金项目
国家重点基础研究发展计划(2011CB100801) （2011CB100801）
中央高校基本科研业务费自主创新重点研究项目(KYZ201114) （KYZ201114）
