动物营养学报2012，Vol.24Issue(5)：859-869,11.DOI:10.3969/j.issn.1006-267x.2012.05.010
大豆异黄酮对奶牛脾脏和肠系淋巴结淋巴细胞干扰素γ、白介素2和白介素4浓度以及雌激素受体β mRNA表达的影响
Effects of Soy Isoflavones on Concentrations of Interferon γ,Interleukin 2 and Interleukin 4 and mRNA Expression of Estrogen Receptor β in Lymphocytes of Spleen and Intestinal Lymph Nodes of Dairy Cows
摘要
Abstract
This paper aims to investigate the effects of soy isoflavones on proliferation, activation and production of cytokines in lymphocytes of spleen and intestinal lymph nodes of dairy cows in vitro. A single factor design was used, and lymphocytes were incubated with different levels of soy isoflavones [ 0. 00 (control) , 0.25, 1.00, 5.00, 25.00 and 100.00 μg/mL] at37℃5% CO2 for 4, 24 and 48 h, respectively. Concentrations of interferon γ, interleukin 2 and interleukin 4 in culture supernatants were determined by double-antibody sandwich enzyme-linked immunosorbent assay, and expression level of estrogen receptors β mRNA in lymphocytes of spleen and intestinal lymph nodes was determined by real-time PCR. The results showed as follows : 1) after incubated with soy isoflavones (0. 25 and 1. 00 μg/mL) for a while, concentrations of interferon y and interleukin 2 in culture supernatants of lymphocytes of spleen were increased at different levels (P < 0. 05 or P <0. 01) , and 48 h later, 5. 00 and 25. 00 μg/mL soy isoflavones reduced interferon γ and interleukin 2 concentrations significantly (P <0.05). 2) In lymphocytes of intestinal lymph nodes, after incubated with soy isoflavones (1. 00 and 5. 00 μg/mL) for a while, concentrations of interferon γ and interleukin 2 were increased at different levels (P < 0. 05 or P < 0. 01); the concentrations tended to be increased by 0.25 μg/mL soy isoflavones (P>0.05) ; 48 h later, concentrations of interferon γ (P<0.05) and interleukin 2 (P <0. 01) were significantly decreased. 3) Compared with that in the control group, interleukin 4 concentration in lymphocytes of spleen in experimental groups were not change significantly (P > 0. 05) , and the concentration in lymph nodes of intestinal lymph nodes in experimental groups was significantly reduced (P < 0. 05 or P < 0. 01). 4) Ratios of interferon 7 and interleukin 4 in lymphocytes of spleen tended to be increased , but that of intestinal lymph nodes tended to be opposite. 5) Expression levels of estrogen receptors β mRNA in lymphocytes of spleen and intestinal lymph nodes were significantly increased (P < 0. 05 or P < 0.01) , and there was positive correlation between levels of estrogen receptors (5 mRNA expression and soy isoflavones. In conclusion, soy isoflavones can increase concentrations of interferon γ and interleukin 2, ratio of interferon γ and interleukin 2, as well as expression level of estrogen receptors β mRNA, but can decrease interleukin 4 concentration; low dose (0. 25 to 5. 00 (μg/mL) soy isoflavones can get better effects on immunity promotion, but high dose (25. 00 to 100.00 (μg/mL) can stimulate expression of estrogen receptors β mRNA关键词
大豆异黄酮/干扰素γ/白介素2/白介素4/雌激素受体β/免疫功能Key words
soy isoflavones/ interferon γ/ interleukin 2 / interleukin 4/ estrogen receptors β/ immunity分类
农业科技引用本文复制引用
王明,孙志刚,刘文奇,杨柳,方洛云,蒋林树..大豆异黄酮对奶牛脾脏和肠系淋巴结淋巴细胞干扰素γ、白介素2和白介素4浓度以及雌激素受体β mRNA表达的影响[J].动物营养学报,2012,24(5):859-869,11.基金项目
国家自然科学基金资助项目(30871796) （30871796）
