南方医科大学学报2012，Vol.32Issue(5)：606-609,4.DOI:10.3969/j.issn.1673-4254.2012.05.003
丙戊酸钠对人肺癌SPC-A1细胞的增殖抑制作用及其机制
Inhibitory effect of sodium valproate on human lung carcinoma SPC-A1 cell proliferation and the mechanism
摘要
Abstract
Objective To observe the effect of sodium valproate (VPA) on the proliferation and apoptosis of human lung carcinoma SPC-A1 cells and the underlying mechanism.Methods The effect of VPA on the proliferation of SPC-A1 cells was evaluated by MTT assay and clone formation assay.Flow cytometry was used to analyze the apoptosis of the cells exposed to VPA.The changes in the expressions of Bcl-xl,Bcl-2,Mcl-1,caspase-9,and caspase-3 in the exposed cells were detected by Western blotting.Results Incubation with VPA for 48 h resulted in a significant inhibition of SPC-A1 cell proliferation,with a IC50 of 1.8 mmol/L VPA treatment also inhibited cell colony formation and induced obvious cell apoptosis.Exposure to 8 mmol/L VPA for 48 h caused a percentage of early apoptotic cells of 60.44%.VPA treatment at different concentrations for 48 h obviously lowered the protein levels of Bcl-xl,Bcl-2,and Mcl-1 and induced caspase-9 and caspase-3 activation in SPC-A1 cells.Conclusion VPA can inhibit the proliferation of SPC-A1 cells by triggering mitochondrion-dependent apoptosis.关键词
丙戊酸钠/凋亡/肺癌/SPC-A1细胞Key words
sodium valproate/apoptosis/lung cancer/SPC-A1 cells分类
医药卫生引用本文复制引用
黄志宏,陈庆,马刘红,陈志明,陈文朴,覃莉,蒋建伟..丙戊酸钠对人肺癌SPC-A1细胞的增殖抑制作用及其机制[J].南方医科大学学报,2012,32(5):606-609,4.基金项目
国家自然科学基金(30772131) （30772131）
广东省科技计划项目(2011B031800012) （2011B031800012）
