南方医科大学学报2012，Vol.32Issue(5)：618-621,4.DOI:10.3969/j.issn.1673-4254.2012.05.006
载多烯紫杉醇脂质微泡增强人胰腺癌BxPC3细胞的增殖抑制和凋亡诱导作用
Enhanced proliferation inhibition and apoptosis-inducing effects of docetaxel-loaded lipid microbubble on human pancreatic cancer cells in vitro
摘要
Abstract
Objective To investigate the proliferation inhibition and apoptosis-inducing effect of docetaxel-loaded lipid microbubble (DLLM) combined with ultrasound targeted microbubble destruction (UTMD) on human pancreatic cancer cells in vitro.Methods DLLM was prepared by mechanical vibration,and its physical properties were characterized.The median inhibitory concentration (IC50) of DLLM was determined with MTT assay,and its cytotoxicity evaluated with cell counting Kit-8 (CCK-8) assay.The effects of docetaxel,DLLM,and DLLM combined with UTMD on cell cycle and apoptosis of BxPC3 cells were tested with flow cytometry (FCM) and TUNEL assay,respectively.Results DLLM had an average size of 1.6 μm,and the average drug entrapment efficiency and drug-loaded amount was 64.2% and 16.1%,respectively.Compared with the control groups,DLLM had a significantly enhanced cytotoxic effect (P<0.01) and caused an increased apoptosis rate in BxPC3 cells (P<0.01).Cell cycle analysis showed that DLLM combined with UTMD resulted in an significantly higher percentage of cells with G2/M phase arrest than the other treatments (P<0.01).Conclusions DLLM combined with UTMD can increase G2/M phase arrest and enhance the proliferation inhibition and apoptosis-inducing effect on BxPC3 cells,and can serve as an effective drug delivery system for pancreatic cancer therapy.关键词
多烯紫杉醇/凋亡/微泡/胰腺癌Key words
docetaxel/apoptosis/microbubbles/pancreatic cancer分类
医药卫生引用本文复制引用
杨健,康娟,曾妍,李奥,吴小翎,王志刚..载多烯紫杉醇脂质微泡增强人胰腺癌BxPC3细胞的增殖抑制和凋亡诱导作用[J].南方医科大学学报,2012,32(5):618-621,4.基金项目
国家自然科学基金(81130025) （81130025）
国家自然科学基金委员会科学部主任基金(81041064) （81041064）
