南方医科大学学报2012，Vol.32Issue(5)：643-646,4.DOI:10.3969/j.issn.1673-4254.2012.05.012
OCT4在膀胱癌中的表达及其临床意义
Expression of OCT4 protein in bladder cancer and its clinicopathological implications
摘要
Abstract
Objective To study the expression of OCT4 protein in bladder cancer and its correlation to the clinicopathologic features and prognosis of bladder cancer.Methods OCT4 mRNA and protein expression was detected in 5 bladder cancer cell lines (RT-4,Tec-Sup,KK47,T24,and 5637) and 1 normal bladder cell lines by real-time PCR and Western blotting,respectively.Immunohistochemical analysis was used to detect the expression of OCT4 protein in 46 bladder cancer samples.Results All the 5 bladder cancer cell lines expressed detectable levels of OCT4 mRNA and proteins,whereas the normal bladder cell line SV-HUC-1 was negative for OCT4 expression.The clinical bladder cancer tissues showed a high positiviry rate of OCT4 expression (76.1%),which was not detected in normal bladder tissues.Specific OCT-4 signals were localized mainly in the nuclei of the cancer cells.The expression rate of OCR protein was significantly higher in bladder cancer tissue than in normal bladder epithelium (P<0.05),and showed a positive correlation to the grade of tumor differentiation and metastasis (P<0.05) but not to the patients' age,gender or TNM stage.Conclusion OCT4 protein expression is associated with tumor differentiation and metastasis in bladder cancer and may play an important role in the early diagnosis and prognostic evaluation of bladder cancer.关键词
膀胱癌/OCT4蛋白/肿瘤转移Key words
bladder cancer/OCT4 protein/cancer metastasis分类
医药卫生引用本文复制引用
赵朋朋,刘春晓,许凯,郑少波,李虎林,徐亚文,徐啊白,李炳坤,黄鹏..OCT4在膀胱癌中的表达及其临床意义[J].南方医科大学学报,2012,32(5):643-646,4.基金项目
国家自然科学基金(81101559) （81101559）
